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The ancient art of dyeing has been used by almost all civilizations of antiquity. Long before the invention of chemical dyes, humanity only used natural sources forobtaining colors. The pleasure of working with natural dyes is that it is never bored and always you can find new sources of color.
Extraction is a separation technique used toremove or separate one compound from a mixture. In the case of pigments or inks this method is used to get the desired color from the natural or synthetic source.
The most commonnatural pigments are chlorophyll (the green color), beta carotene the (orange color) and lycopene (the red color).
Figure 1. Show the molecules of a,b and d chlorophyll, Beta carotene and Lycopene.

Learning Objectives
Extract the colorfull pigments of the different plants to be analyzed by chromatography. Record thedifferent color composition for every sample.

1. Fruits, Three Leaves and Flowers
2. Mortar and Pestle
3. Acetone, Ethanol and Water as Solvents
4.Filters and Filter Paper
5. Beakers

1. Obtain a mortar and pestle and choose a plant for your extraction.

2. Add the plant to the mortar and then crushyour plant in the mortar with the pestle.


3. Crush as much as you can and then add the solvent (Water, Acetone and or Ethanol).


4. Filter the extract toremove all non-soluble plant material.


5. Leave the beaker in the hood to evaporate the acetone.


6. After the acetone evaporation add just the enoughacetone to the beaker until have a high concentrated solution.

7. Use the TLC and or the Paper chromatography to separate those pigments using the best solvent as a mobile phase.
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