Cytokeratina 5

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  • Publicado : 3 de marzo de 2011
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Cytokeratin 5 (CK5)
Concentrated and Prediluted Monoclonal Antibody
Control Number: 901-234-091506 Catalog Number: Description: Dilution: Diluent: CM 234 A, B, C 0.1, 0.5, 1.0 ml, concentrated 1:50 -1:100 Van Gogh Yellow PM 234 AA 6.0 ml, prediluted Ready-to-use N/A

ISO 9001:2000 CERTIFIED

Pretreatment Protocol cont'd: Allow solution to cool for 20 minutes then wash in distilled water.Avidin-Biotin Block is recommended: 1. Apply Avidin solution for 10 minutes and wash with TBS wash buffer. 2. Apply Biotin solution for 10 minutes and wash with TBS wash buffer. Protein Block: Incubate for 10-15 minutes at RT with BIOCARE's Background Sniper. Primary Antibody: Incubate for 30 minutes at RT. Link: Incubate for 10 minutes at RT with a link Label: Incubate for 10 minutes at RT with alabel. Chromogen: Incubate for 5 minutes at RT when using BIOCARE's DAB. - OR - Incubate for 10 minutes at RT when using BIOCARE's Vulcan Fast Red. Technical Note: This antibody has been standardized with BIOCARE's 4 plus detection system. It can also be used on an automated staining system and with other BIOCARE polymer detection kits. Use TBS buffer for washing steps. Performance Characteristics:The optimum antibody dilution and protocols for a specific application can vary. These include, but are not limited to: fixation, heat-retrieval method, incubation times, tissue section thickness and detection kit used. Due to the superior sensitivity of these unique reagents, the recommended incubation times and titers listed are not applicable to other detection systems, as results may vary.The data sheet recommendations and protocols are based on exclusive use of BIOCARE products. Ultimately, it is the responsibility of the investigator to determine optimal conditions. These products are tools that can be used for interpretation of morphological findings in conjunction with other diagnostic tests and pertinent clinical data by a qualified pathologist. Quality Control: Refer to NCCLSQuality Assurance for Immunocytochemistry approved guidelines, December 1999 MM4-A Vol.19 No.26 for more information about Tissue Controls. Precautions: This antibody contains less than 0.1% sodium azide. Concentrations less than 0.1% are not reportable hazardous materials according to U.S. 29 CFR 1910.1200, OSHA Hazard communication and EC Directive 91/155/EC. Sodium azide (NaN3) used as apreservative is toxic if ingested. Sodium azide may react with lead and copper plumbing to form highly explosive metal azides. Upon disposal, flush with large volumes of water to prevent azide build-up in plumbing. (Center for disease control, 1976, National Institute of Occupational Safety and Health, 1976) Specimens, before and after fixation and all materials exposed to them, should be handled as ifcapable of transmitting infection and disposed of with proper precautions. Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with reagents and specimens. If reagents or specimens come in contact with sensitive areas, wash with copious amounts of water. Microbial contamination of reagents may result in an increase in nonspecific staining. Incubation times ortemperatures other than those specified may give erroneous results. The user must validate any such change. The MSDS is available upon request. Troubleshooting: Follow the antibody specific protocol recommendations according to data sheet provided. If atypical results occur, contact BIOCARE's Technical Support at 1-800-542-2002. Limitations and Warranty: There are no warranties, expressed or implied,which extend beyond this description. BIOCARE is not liable for property damage, personal injury, or economic loss caused by this product.

Intended Use: For In Vitro Diagnostic Use Summary and Explanation: CK5 is a 58kD protein that is closely related to CK6. ELISA was carried out to screen for antibodies that were positive for CK5 and negative with CK6. Results showed that the XM26 clone was...
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