An LC/MS/MS Method for the Simultaneous Determination of Diazepam and its Metabolites in Fly Larvae Sampled From Diazepam Spiked Porcine Liver
Analytical Unit St Georges - University of London,London, UK
Rebecca Beauchamp, Alan Beeby, Heidi Sanderson, Jennifer Button, Terry Lee and David W Holt

Forensic entomology is a recognised method of estimating post-mortem interval, butrelatively little research has examined the use of larvae in forensic toxicology. Forensic entomotoxicology includes the study of the effects of drugs and toxins on the development rate ofcarrion-feeding insects, and the use of these as alternative sample matrices in the absence of other samples. Analysis of living material, such as larvae, offers a number of technical advantages for drugdetection over putrefied human remains. The extraction of drugs from larvae is the same as that from tissue, however, no emulsion is formed, whereas this is not always the case with human tissue. There isalso less contamination observed from endogenous substances, which is particularly problematic with putrefied human remains. Larvae are usually present in abundance on decomposed bodies and sampling isoften a relatively straight forward procedure. To date, most forensic entomotoxicological studies have concentrated on opiates rather than commonly prescribed drugs, such as benzodiazepines. LC/MS/MSis becoming widely used in toxicology laboratories and is an established technique for screening and quantifying benzodiazepines. A rapid, sensitive and selective LC/MS/MS method for theidentification of diazepam and its metabolites; desmethyldiazepam, oxazepam and temazepam in liver and fly larvae sampled from spiked porcine liver is described. Diazepam, first approved for usage in the early1960s, is one of the most frequently prescribed drugs of the benzodiazepine group. Its uses include; treatment of anxiety and anxiety related insomnia, muscle relaxant, anti-epileptic and pre-operative...