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Acute effects of Benzo[a]pyrene, anthracene and a fuel oil on biomarkers of the common goby Pomatoschistus microps (Teleostei, Gobiidae) ☆
L.R. Vieiraa,b,⁎, A. Sousaa , M.F. Frascoa,c , I.Limaa,c , F. Morgadob , L. Guilherminoa,c
CIMAR-LA/CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Laboratório de Ecotoxicologia, Rua dos Bragas, 289, 4050-123 Porto, Portugal b CESAM & Departamento de Biologia, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal c ICBAS - Instituto de Ciências Biomédicas de AbelSalazar, Universidade do Porto, Departamento de Estudos de Populações, Laboratório de Ecotoxicologia, Lg. Prof. Abel Salazar, 2, 4099-003 Porto, Portugal
AR TIC LE I N FO
Article history: Received 30 July 2007 Received in revised form 30 December 2007 Accepted 31 January 2008 Available online 17 March 2008 Keywords: Pomatoschistus microps Acetylcholinesterase Lactate dehydrogenase GlutathioneS-transferases Anti-oxidant enzymes Benzo[a]pyrene Anthracene Fuel oil
ABS TR ACT
The objective of this study was to investigate the effects of two different PAHs and a complex petrochemical mixture on the common goby, Pomatoschistus microps, using selected biomarkers as effect criteria. Benzo[a]pyrene (BaP) and anthracene were used as reference substances, while the water accommodated fractionof #4 fuel-oil (#4 WAF) was used as an example of a petrochemical mixture. P. microps was used since it is both a suitable bioindicator and a good test organism. Groups of fish were exposed to different concentrations of each of the test substances for 96 h and the activities of several enzymes commonly used as biomarkers were determined at the end of the bioassays. All the substances inhibited P.microps acetylcholinesterase (AChE) indicating that they have at least one mechanism of neurotoxicity in common: the disruption of cholinergic transmission by inhibition of AChE. An induction of lactate dehydrogenase (LDH) activity was found in fish exposed to BaP or to anthracene, suggesting an increase of the anaerobic pathway of energy production. On the contrary, inhibition of LDH was found infish exposed to #4 WAF, suggesting a distinct effect of the mixture. An induction of P. microps glutathione S-transferase (GST) activity was found in fish exposed to BaP or to #4 WAF, while an inhibition was observed after exposure to anthracene. These results suggest that GST is involved in the detoxification of BaP and #4 WAF, but not of anthracene. All the substances increased catalaseactivity and isolated PAHs also increased superoxide dismutase, glutathione reductase and glutathione peroxidase activities, while #4 WAF did not cause significant alterations on these enzymes. These results suggest that all the substances may induce oxidative stress on P. microps, with BaP and anthracene apparently having more oxidative stress potential than #4 WAF. © 2008 Elsevier B.V. All rightsreserved.
☆ This work was supported by “Fundação para a Ciência e a Tecnologia” (FCT) from Portugal, through PhD grants (SFRH/BD/17118/2004/ 59R5, SFRH/BD/6826/2001, SFRH/BD/13163/2003) and the project “RISKA” (Contract: POCTI/BSE/46225/2002). ⁎ Corresponding author. Departamento de Biologia, Campus Universitário de Santiago, Unversidade de Aveiro 3810-193 Aveiro. Tel.: +351 234 370350; fax: +351234 426408. E-mail address: Bioluis@bio.ua.pt (L.R. Vieira).
0048-9697/$ – see front matter © 2008 Elsevier B.V. All rights reserved. doi:10.1016/j.scitotenv.2008.01.052
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