1957 12: 657-670
A Comparative Evaluation of the Sensitivity of the L.E. Cell Test Performed Simultaneously by Different Methods
EDMUND L. DUBOIS and VIVIAN FREEMAN
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Comparative L.E. Cell
Evaluation Test Performed by Different
I.1. DtBoIs descriptioms of this its
the SensitivitySimultaneously Methods
for the As each
the has-c reports methods, of the
performance technic In view merits of appeared
described that their
sarious is-as to deterto perfornis blood antico-
proponent-sthe most mine the
sensitive. comparative a one
of the conflicting of the various different and and
and the necessity it si-as decided
simultaneously draivn from agulants,
L.E. tests on peripheral relative importance of on the L.E.
thissttndy the following four tecisusics were utilized after blood from a sirsgle venipunct.ure: 10 ml. was added to each of two test tubes containing 0.75 and 3.75 mg. of aqueous sodiunns heparin, another 10 cc. was permitted to clot, andfinally several drops were used to perform the Snapper-Nathan
Dtiring the taming 30 cc.
first phase of peripheral
10 mg. per ml.
be approxinsated by
mg. per cc. obtained
through a 21 gauge needle. The preservative contained in the 50 mg. heparin was phenol 0.5% and in the 10 mg. concentration was chlorobutanoh 0.5%. Outlined below is the method by which we performed the test utilizingheparinsized blood. 1) The heparinized blood was allowed to remain at room temperature 30-60 nun. 2) The specimen was then centrifuged at approxinuately 1000 RPM or (104 X G) for
placed or into (104 X G)
a Wintrobe for
5) unntil 6)
The Wintrobe a separation The supernatant
tube was centrifuged 1000 at RPM the three layers was obtained. plasma was again removed
ous glass to
buffy coat was carefully aspirated into the pipetandthen slides. In order to make a thin sections of the smear, the smearing slide was and then pulled backward over one-half width the of the blood film. This better study the cytology when the btnffy coat was very in leukocytes. rids 8) The preparations were stained with Wright’s stain in the usual manner. 9) The film was examined especially at the edges for at least 10 minutes mersion lens. In...