There are two roads connecting Los Osos to other communities: South BayBoulevard, which leads to Morro Bay via Highway 1, and Los Osos Valley Road, which leads to San Luis Obispo. Inclement weather and road construction occasionally forces the closure of aroute, possibly requiring detours to arrive at one's destination. This has been much less frequent since the Chorro Bridge replaced the Twin Bridges on South Bay Boulevard.
Los Osos servesas the entrance to Montaña de Oro State Park. Los Osos Valley Road reaches the coast at the south end of Estero Bay and continues south into the state park. Morro Bay State Park bordersthe northeast of the town. South Bay Boulevard travels through the middle of the park after it leaves Los Osos. Los Osos is also home to the Elfin Forrest  which sits on the southeastside of the estuary that sits between Los Osos and Morro Bay State Park.
Large groves of non-native eucalyptus trees attract the annually migrating Monarch butterflies to Los Osos.Los Osos' proximity to the Diablo Canyon Power Plant means that warning sirens are located throughout the town so that the residents will be warned if the power plant should suffer ameltdown or other adverse event. The sirens are also found in other cities nearby, including Morro Bay, San Luis Obispo and Avila Beach. Except for yearly tests, the sirens go largely unused.