Pach clamping

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Patch Clamping: An Introductory Guide To Patch Clamp Electrophysiology. Areles Molleman Copyright 2003 John Wiley & Sons, Ltd. ISBN: 0-471-48685-X

Patch Clamping

Patch Clamping
An Introductory Guide to Patch Clamp Electrophysiology

Areles Molleman
University of Hertfordshire, UK

Copyright # 2003

John Wiley & Sons Ltd, The Atrium, Southern Gate, Chichester, West Sussex PO19 8SQ,England Telephone (+44) 1243 779777

Email (for orders and customer service enquiries): cs-books@wiley.co.uk Visit our Home Page on www.wileyeurope.com or www.wiley.com All Rights Reserved. No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, scanning or otherwise, except underthe terms of the Copyright, Designs and Patents Act 1988 or under the terms of a licence issued by the Copyright Licensing Agency Ltd, 90 Tottenham Court Road, London W1T 4LP, UK, without the permission in writing of the Publisher. Requests to the Publisher should be addressed to the Permissions Department, John Wiley & Sons Ltd, The Atrium, Southern Gate, Chichester, West Sussex PO19 8SQ, England,or emailed or permreq@wiley.co.uk, or faxed to (+44) 1243 770571. This publication is designed to provide accurate and authoritative information in regard to the subject matter covered. It is sold on the understanding that the Publisher is not engaged in rendering professional services. If professional advice or other expert assistance is required, the services of a competent professional shouldbe sought. Other Wiley Editorial Offices John Wiley & Sons Inc., 111 River Street, Hoboken, NJ 07030, USA Jossey-Bass, 989 Market Street, San Francisco, CA 94103-1741, USA Wiley-VCH Verlag GmbH, Boschstr. 12, D-69469 Weinheim, Germany John Wiley & Sons Australia Ltd, 33 Park Road, Milton, Queensland 4064, Australia John Wiley & Sons (Asia) Pte Ltd, 2 Clementi Loop #02-01, Jin Xing Distripark,Singapore 129809 John Wiley & Sons Canada Ltd, 22 Worcester Road, Etobicoke, Ontario, Canada M9W 1L1

Library of Congress Cataloging-in-Publication Data Molleman, Areles. Patch clamping : an introductory guide to patch clamp electrophysiology / Areles Molleman. p. cm. Includes bibliographical references and index. ISBN 0 471 48685 X 1. Patch-clamp techniques (Electrophysiology) I. Title. QP341.M6842002 572’.437 — dc21 2002027413 British Library Cataloguing in Publication Data A catalogue record for this book is available from the British Library ISBN 0 471 48685 X Paperback Typeset in 11/131 Sabon by Keytec Typesetting Ltd 2 Printed and bound in Great Britain by TJ International, Padstow, Cornwall This book is printed on acid-free paper responsibly manufactured from sustainable forestry, inwhich at least two trees are planted for each one used for paper production.

Contents

Preface 1
1.1

ix 1
1

Introduction
Patch Clamping and its Context

2
2.1

Basic Theoretical Principles
Introduction to Membrane Biology
2.1.1 2.1.2 2.1.3 2.1.4 The plasma membrane and its ionic environment Electrochemical gradients and the Nernst equation Maintenance of ion gradients and themembrane potential Ion channels Driving force and membrane resistance Membrane capacitance Consequences of membrane capacitance An electronic model of the plasma membrane The basics of equivalent circuits Intracellular recording Voltage clamp and current clamp Introduction to patch clamp configurations The equivalent circuit for the cell-attached patch configuration The equivalent circuit for thewhole-cell configuration The equivalent circuit for the excised patch configurations

5
5
5 7 8 11

2.2

Electrical Properties of the Cell Membrane
2.2.1 2.2.2 2.2.3 2.2.4

13
13 15 16 17

2.3

Recording Modes and their Equivalent Circuits
2.3.1 2.3.2 2.3.3 2.3.4 2.3.5 2.3.6 2.3.7

18
18 22 28 30 35 39 40

3
3.1

Requirements
The Platform
3.1.1 3.1.2 3.1.3 Stability:...
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