Passive oral immunization by egg yolk immunoglobulin (igy) to vibrio cholerae effectively prevents cholera

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Passive Oral Immunization by Egg Yolk Immunoglobulin (IgY) to Vibrio cholerae Effectively Prevents Cholera
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    Reactivity of IgY to V. cholerae and cholera toxin Antigen IgY Concentration 10 µg ml 10 µg ml 106 CFU ml 100 µg ml  1 µgml 100 µg ml  1 µg ml 106 CFU ml 100 µg ml  1 µg ml 100 µg ml  1 µg ml 106 CFU ml 100 µg ml  1 µg ml 100 µg ml  1 µg ml 1.430 0.103 1.588 0.220 0.161 0.010 0.100 0.007 2.908 0.743 3.000 1.229 0.392 0.040 0.183 0.040 1.165 0.086 1.438 0.128 0.132 0.013 0.088 0.087 2.460 0.656 3.000 0.874 0.538 0.060 0.040 0.259 1.641 0.103 1.702 0.220 0.174 0.010 0.071 0.018 3.000 0.734 3.000 0.944 0.100 0.0350.125 0.020 Concentration 100 µg ml  1 µg ml 100 µg ml  1 µg ml Control IgY OD 0.139 0.044 0.065 0.038 0.004 0.008 0.002 0.002 Anti VC IgY OD 0.069 0.036 0.069 0.035 0.005 0.001 0.003 0.002

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Source Cholera Toxin Cholera toxin B subunit

Anti CTB IgY OD 3.000 0.660 3.000 0.839 0.105 0.054 0.123 0.082

V. cholerae O1 E1 Tor (inaba) 7.5 heat shock formalin V. cholerae O1 E1 Tor (ogawa)7.5 heat shock formalin V. cholerae O139      7.5 heat shock formalin

0.592 0.106 0.812 0.167

0.014 0.054 0.027 0.017

0.421 0.065 0.709 0.108

0.105 0.010 0.186 0.049

0.582 0.075 0.884 0.173

0.094 0.003 0.032 0.004

 

 

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  Effect of anti CTB IgY on elongation of CHO cells. A, Morphology of theCHO cells. CHO cells were cultured in 24 well dishes (1). CT treatment changed the cell morphology (2). This morphological change was inhibited by preincubation of CT with 15 µg ml anti CTB IgY (3); B, Effect of different amounts of IgY on elongation. CT was treated with different amounts of anti CTB IgY and then reacted to the CHO cells. The percentage of round cells to total cells was obtained byaveraging the counts obtained by 10 microscope view fileds.

 

 

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  Therapeutic effect of IgY on cholera in infant mice. Four day old suckling mice weregastrointestinally inoculated with 50 µl of O1 or O139 cells (7.5 108 CFU ml). After 3 hr, the mice were then administered 50 µl of anti V.C IgY, anti CTB IgY, or a mixture of 1 mg ml of each at different intervals. A, Inoculation of V. cholerae O1 El Tor (Inaba). The mice were then administered the following at 2 h intervals: PBS, (●); control IgY, (■) or anti CTB IgY, (△); anti V.C IgY, (◇); or both anti CTB IgYand anti V.C IgY, ( ); B, Inoculation with V. cholerae O139. The mice were then administered the following at 2 h intervals: PBS, (●); anti CTB IgY, (■); and anti V.C IgY, (△); C, Effects of different intervals. After the challenge with V. cholerae O1 El Tor (Inaba), the mice were administered with PBS, (●); or a mixture of anti V.C and CTB IgYs at intervals of 2 h, (■); 4 h, (△); 6 h, ( ); 8 h,(○); or 12 h, (□).

 

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  Protective effect of IgY. A, The simultaneous administration of cells and IgY. O1 (Inaba) or O139 cells were mixed with anti V. C IgY and then injected into the stomach of thesuckling mice. (1) O1 cells alone, (●); O1 cells with anti V.C IgY 0.5 mg ml, (□); 1 mg ml, (△); or 2 mg ml, ( ) . (2) O139 cells alone, (●); O139 cells with anti V.C IgY 1 mg ml, (■); B, Effect of pretreat ment with IgYs. The suckling mice were first administered PBS, (●); or 1 mg ml of anti V.C and CTB mixed IgYs, (■). After 1 h, 50 µl (7.5 108 CFU ml) of O1 organisms was challenged. After 2 h, the...
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