Periodoncia

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  • Publicado : 27 de septiembre de 2010
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Effects of Current Provisional Restoration Materials on the Viability of Fibroblasts
Mustafa Ulker,a H. Esra Ulker,b Mustafa Zortuk,c Mehmet Bulbul,d Ali Riza Tuncdemir,e and M. Selim Bilgine
aDDS,PhD, Assistant Professor, Erciyes University, Faculty of Dentistry, Department of Conservative Dentistry, Kayseri, Turkey
bDDS, PhD, Research Assistant, Selcuk University, Faculty of Dentistry,Department of Conservative Dentistry, Konya, Turkey
cDDS, PhD, Assistant Professor, Erciyes University, Faculty of Dentistry, Department of Prosthetic Dentistry, Kayseri, Turkey
dDDS, PhD, Specialist inOral Health Hospital, Selcuklu, Konya, Turkey
eDDS, PhD, Selcuk University Faculty of Dentistry, Department of Prosthetic Dentistry, Konya, Turkey
Corresponding author: Dr. Mustafa Ulker, ErciyesUniversity, Faculty of Dentistry, Department of Conservative Dentistry, 38039, Campus, Kayseri, Turkey. Phone: + 90 352 4374937-29129 Fax: + 90 352 4380657, E-mail: muulker@hotmail.com or ; Email:mulker@selcuk.edu.tr
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Abstract
Objectives:
The aim of the present study was to evaluate the cytotoxic effects of three different provisional restoration materials on fibroblasts. Twobis-acrylic based [Tempofit Duomix (Detax), Protemp 3 Garant (3M ESPE)] and one urethan dimethacrylate [Revotek LC (GC Corporation)] based provisional restoration materials used.
Methods:
Materialswere prepared according to the manufacturers’ instructions in standard teflon disks (2×5 mm) and four samples were extracted in 7 ml of Basal Medium Eagle with 10% new born calf serum and 100 mg/mlpenicillin/streptomycin for 24 hours. The L929 fibroblast cells were plated (25.000 cells/ml) in well plates, and maintained in a CO2 incubator at 37°C for 24h. After 24 hours, the incubation mediumwas replaced by the immersed medium in which the samples were stored and the L929 fibroblasts were incubated in contact with eluates for 24 hours at 37°C for 24h. The fibroblast cell viability was...
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