Pracicas de biorreactores

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McMaster University, Canada

Y | * World Scientific

Published by World Scientific Publishing Co. Pte.Ltd. 5 Toh Tuck Link, Singapore 596224 USA office: 27 Warren Street, Suite 401-402, Hackensack, NJ 07601 UK office: 57 Shelton Street, Covent Garden, London WC2H 9HE

British Library Cataloguing-in-Publication Data A catalogue record for this book is available from the British Library.

Cover: Image courtesy of Millipore Corporation.

PRINCIPLES OF BIOSEPARATIONS ENGINEERING Copyright © 2006by World Scientific Publishing Co. Pte. Ltd. All rights reserved. This book, or parts thereof, may not be reproduced in any form or by any means, electronic or mechanical, including photocopying, recording or any information storage and retrieval system now known or to be invented, without written permission from the Publisher.

For photocopying of material in this volume, please pay a copyingfee through the Copyright Clearance Center, Inc., 222 Rosewood Drive, Danvers, MA 01923, USA. In this case permission to photocopy is not required from the publisher.

ISBN 981-256-892-1

Printed in Singapore by World Scientific Printers (S) Pte Ltd

To my wife, Sutapa

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The author acknowledges with thanks the assistanceprovided by the individuals and organisations named below: Christine Zardecki of RCSB Protein Data Bank for kind permission to use images of protein and DNA structures (Figures 2.1, 2.2 and 2.3 of this book) Stanley Goldberg and Marc Lustig of Glen Mills Inc. and Andre Wrysch W. A. of Bachofen AG for providing me with Figures 4.5, 4.10 and 4.11 of this book and for their enthusiastic support for thisproject Dominic Spricigo of IKA® -WERKE GmbH & Co. KG for providing me with Figure 4.7 of this book Kevin McLaughlin of Shimadzu Scientific Instruments for providing me with Figure 9.4 of this book Gene Lauffer of Menardi for kind permission to use images of filtration devices from their website (Figures 10.7 and 10.10 of this book) Dana Hubbard, Susan Cheah and Shannon Meirzon of MilliporeCorporation for providing me with Figures 11.4 (A, B and C), 11.12, 11.13 and 11.14 of this book PCI Membranes for providing me with Figure 11.15 of this book Shuichi Ogawa of Asahi Kasei for kind permission to use image of membrane filtration device from their website (Figure 11.16 of this book) Patricia Stancati of Sartorius for providing me with Figure 11.39 of this book Sandra Covelli and MichaelKilleen of Pall Corporation for providing me with Figure 11.41 of this book


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Bioseparations engineering refers to the systematic study of the scientific and engineering principles utilized for large-scale purification of biological products: biopharmaceuticals, biochemicals, foods, nutraceuticals and diagnostic reagents. Bioseparationsengineering, both as an academic topic as well as an industrial practice has undergone significant growth and increase in importance in the past decade. Its entry into academia as a taught course took place as usually happens with any new topic: at the postgraduate level. It is now taught as a regular course in many undergraduate chemical and biomolecular engineering programs. It is also taught eitheras a separate course or indeed as a component of bioprocess engineering in almost all undergraduate and postgraduate programs in biotechnology. Most projects start with some necessity and this book is no exception. In the year 1999, as a new faculty member at Oxford University I was assigned to teach the relatively new C5A Bioprocess Engineering and C5B Separations courses for undergraduate...
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