Punto isoelectrico

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  • Publicado : 13 de noviembre de 2011
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CH3COOH + H2O CH3COO¯ + H3O

TUBO 1
CiVi = CfVf
Cf=CiViVf

Cf=0.01N∙0.6210

Cf=6.1999x10-4

pH=pka+log[A-][AH]
pH=4.74+log0.16.199x10-4

pH=5.94
TUBO 2CiVi = CfVf
Cf=CiViVf

Cf=0.01N∙1.2510

Cf=1.2499x10-3

pH=pka+log[A-][AH]
pH=4.74+log0.11.2499x10-3

pH=5.64
TUBO 3

CiVi = CfVf
Cf=CiViVfCf=0.1N∙0.2510

Cf=2.5x10-4

pH=pka+log[A-][AH]
pH=4.74+log0.12.5x10-4

pH=5.34
TUBO 4

CiVi = CfVf
Cf=CiViVf

Cf=0.1N∙0.510

Cf=0.005

pH=pka+log[A-][AH]pH=4.74+log0.10.005

pH=6.04
TUBO 5

CiVi = CfVf
Cf=CiViVf

Cf=0.1N∙110

Cf=0.01

pH=pka+log[A-][AH]
pH=4.74+log0.10.01

pH=5.74
TUBO 6

CiVi = CfVfCf=CiViVf

Cf=0.1N∙210

Cf=0.02

pH=pka+log[A-][AH]
pH=4.74+log0.10.02

pH=5.43
TUBO 7

CiVi = CfVf
Cf=CiViVf

Cf=0.1N∙410

Cf=0.04pH=pka+log[A-][AH]
pH=4.74+log0.10.04

pH=5.13
TUBO 8

CiVi = CfVf
Cf=CiViVf

Cf=0.1N∙810

Cf=0.08

pH=pka+log[A-][AH]
pH=4.74+log0.10.08

pH=4.83
TUBO 9

CiVi= CfVf
Cf=CiViVf

Cf=1N∙1.610

Cf=0.16

pH=pka+log[A-][AH]
pH=4.74+log0.10.16

pH=4.53

Tubo No. | pH | Ac. Acético 0,01N (ml) | Agua destilada | Ac.Acético 0.1N (ml) | Ac. Acético 1N (ml) | Caseína en acetato de sodio (ml) | Observaciones |
1 | 5.94 | 0.62 | 8.38 | --- | --- | 1.0 | |
2 | 5.64 | 1.25 | 7.75 | --- |--- | 1.0 | |
3 | 5.34 | --- | 8.75 | 0.25 | --- | 1.0 | |
4 | 6.04 | --- | 8.5 | 0.5 | --- | 1.0 | |
5 | 5.74 | --- | 8.0 | 1.0 | --- | 1.0 | |
6 | 5.43 |--- | 7.0 | 2.0 | --- | 1.0 | |
7 | 5.13 | --- | 5.0 | 4.0 | --- | 1.0 | |
8 | 4.83 | --- | 1.0 | 8.0 | --- | 1.0 | |
9 | 4.53 | --- | 7.4 | --- | 1.6 | 1.0 | |
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