Test elisa de estradiol

Solo disponible en BuenasTareas
  • Páginas : 8 (1940 palabras )
  • Descarga(s) : 0
  • Publicado : 26 de agosto de 2010
Leer documento completo
Vista previa del texto
DIAGNOSTIC AUTOMATION, INC.
23961 Craftsman Road, Suite D/E/F, Calabasas, CA 91302 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com
See external label 2°C-8°C Σ=96 tests Cat # 2046Z

Estradiol
Cat # 2046Z Enzyme Immunoassay for the Quantitative Determination of Estradiol (E2) Concentration in Human Serum or Plasma Test MethodPrinciple Detection Range Sample Specificity Sensitivity Total Time Shelf Life Estradiol (E2) ELISA ELISA: Enzyme Linked Immunosorbent Assay Indirect: Antigen Coated 0-1000 pg/ml 25ul serum 100% 10pg/ml ~ 110 min 12-14 months

* Laboratory results can never be the only base of a medical report. The patient history and further tests have to be taken into account.

DAI Code # 18

1

INTENDEDUSE
For the quantitative determination of Estradiol (E2) concentration in human serum

INTRODUCTION
Estradiol (E2) is a C18 steroid hormone with a phenolic A ring. This steroid hormone has a molecular weight of 272.4. It is the most potent natural Estrogen, produced mainly by the ovary, placenta, and in smaller amounts by the adrenal cortex, and the male testes (1,2,3). Estradiol (E2) issecreted into the blood stream where 98% of it circulates bound to sex hormone binding globulin (SHBG). To a lesser extent it is bound to other serum proteins such as albumin. Only a tiny fraction circulates as free hormone or in the conjugated form (4,5). Estrogenic activity is effected via estradiol-receptor complexes which trigger the appropriate response at the nuclear level in the target sites.These sites include the follicles, uterus, breast, vagina, urethra, hypothalamus, pituitary and to a lesser extent the liver and skin. In non-pregnant women with normal menstrual cycles, estradiol secretion follows a cyclic, biphasic pattern with the highest concentration found immediately prior to ovulation (6,7). The rising estradiol concentration is understood to exert a positive feedback influenceat the level of the pituitary where it influences the secretion of the gonadotropins, follicle stimulating hormone (FSH), and luteinizing hormone (LH), which are essential for follicular maturation and ovulation, respectively (8,9). Following ovulation, estradiol levels fall rapidly until the luteal cells become active resulting in a secondary gentle rise and plateau of estradiol in the lutealphase. During pregnancy, maternal serum Estradiol levels increase considerably, to well above the pre-ovulatory peak levels and high levels are sustained throughout pregnancy (10). Serum Estradiol measurements are a valuable index in evaluating a variety of menstrual dysfunctions such as precocious or delayed puberty in girls (11) and primary and secondary amenorrhea and menopause (12). Estradiollevels have been reported to be increased in patients with feminizing syndromes (14), gynaecomastia (15) and testicular tumors (16). In cases of infertility, serum Estradiol measurements are useful for monitoring induction of ovulation following treatment with, for example, clomiphene citrate, LH-releasing hormone (LH-RH), or exogenous gonadotropins (17, 18). During ovarian hyperstimulation for invitro fertilization (IVF), serum estradiol concentrations are usually monitored daily for optimal timing of human chorionic gonadotropin (hCG) administration and oocyte collection (19).

PRINCIPLE OF THE TEST
The E2 EIA is based on the principle of competitive binding between E2 in the test specimen and E2HRP conjugate for a constant amount of rabbit anti-Estradiol. In the incubation, goatanti-rabbit IgGcoated wells are incubated with 25 µl E2 standards, controls, patient samples, 100 µl Estradiol-HRP Conjugate Reagent and 50 µl rabbit anti-Estradiol reagent at room temperature (18-25°C) for 90 minutes. During the incubation, a fixed amount of HRP-labeled E2 competes with the endogenous E2 in the standard, sample, or quality control serum for a fixed number of binding sites of the...
tracking img