Vias metabolicas

TIME-COURSE OF CHANGES IN OXIDATIVE STRESS AND ANTIOXIDANT STATUS RESPONSES FOLLOWING A SOCCER GAME
IOANNIS G. FATOUROS,1 ATHANASIOS CHATZINIKOLAOU,1 IOANNIS I. DOUROUDOS,1 MICHALIS G. NIKOLAIDIS,2 ANTONIOS KYPAROS,3 KONSTANTINOS MARGONIS,1 YIANNIS MICHAILIDIS,1 ANTONIOS VANTARAKIS,1 KYRIAKOS TAXILDARIS,1 IOANNIS KATRABASAS,4 DIMITRIOS MANDALIDIS,5 DIMITRIOS KOURETAS,3 AND ATHANASIOS Z.JAMURTAS2,6
1

Department of Physical Education and Sport Science, Democritus University of Thrace, Komotini, Greece; 2Institute of Human Performance and Rehabilitation, Centre for Research and Technology—Thessaly (CERETETH), Trikala, Greece; 3Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece; 4Department of Orthopedics, Asklipieion General Hospital, Voula, Greece;5Department of Physical Education and Sport Science, University of Athens, Athens, Greece; and 6 Department of Physical Education and Sport Science, University of Thessaly, Trikala, Greece

ABSTRACT
Fatouros, IG, Chatzinikolaou, A, Douroudos, II, Nikolaidis, MG, Kyparos, A, Margonis, A, Michailidis, Y, Vantarakis, A, Taxildaris, K, Katrabasas, I, Mandalidis, D, Kouretas, D, and Jamurtas, AZ.Time-course of changes in oxidative stress and antioxidant status responses following a soccer game. J Strength Cond Res 24(12): 3278–3286, 2010—Exercise-induced muscle damage is associated with an acute-phase inflammatory response characterized by phagocyte infiltration into muscle and free radical production. Although soccer includes intense eccentric muscle actions that cause muscle damage, theoxidative stress responses after a soccer game are currently unknown. The present investigation attempted to determine the responses of circulating levels of oxidative stress and antioxidant status markers during recovery from a soccer game. Twenty soccer players (experimental group) were assigned to 2 different teams that competed against each other (2 3 45 minutes). Ten other players served ascontrols (rested). Creatine kinase (CK) activity, uric acid, leukocyte count, malondialdehyde (MDA), protein carbnyls (PC), reduced (GSH) and oxidized glutathione (GSSG), antioxidant capacity (TAC), catalase, glutathione peroxidase activity (GPX), delayedonset of muscle soreness (DOMS), and anaerobic performance (speed, vertical jump performance) were measured before and following (immediately post, 24hours, 48 hours, 72 hours) the game. Performance deteriorated (2–17%, p , 0.05) throughout recovery. Leukocytosis developed (p , 0.05) immediately

following the game and at 24 hours. Both CK and DOMS (3–8fold, p , 0.05) increased from baseline and remained elevated (p , 0.05) through 48 hours. Thiobarbituric acid reactive substances (TBARS), PC, uric acid, GPX, and TAC increased (13–67%, p ,0.05) throughout recovery, whereas catalase was elevated (38%, p , 0.05) only immediately after the game. GSH/GSSG declined (17–75%, p , 0.05) throughout recovery. Our results suggest that oxidative stress is markedly upregulated by a soccer game, probably as a part of the exercise-induced inflammatory response, and is accompanied by a marked deterioration of anaerobic performance for as long as 72hours.

KEY WORDS free radicals, antioxidant status, soccer, exerciseinduced inflammation, muscle soreness

INTRODUCTION

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Address correspondence to Ioannis G. Fatouros, ifatouro@phyed.duth.gr. 24(12)/3278–3286 Journal of Strength and Conditioning Research Ó 2010 National Strength and Conditioning Association

eactive oxygen species (ROS) are continuously produced during cellular oxygenmetabolism and they have been closely linked to the pathophysiology of a vast number of chronic diseases and aging (12). Although ROS are important signalling molecules in various cellular pathways, marked changes in redox status caused by elevated ROS generation may be detrimental to cellular homeostasis and function because they induce oxidative modifications to macromolecules such as lipids,...