Acondicionamiento de columnas HPLC

Equilibrar, Instalar y Almacenar Columnas HPLC

COLUMN EQUILIBRATION
Proper column equilibration is time well spent as it will give you more consistent result and reduce trouble-shotting. This isof special importance with LiChroCART cartridges, because the columns are not closed and can dry out easily. LiChroCART cartridges need to be flushed properly before use.
Verify that your mobilephase is miscible with the shipping solvent before starting to flush or equilibrate the column.
COLUMN INSTALLATION
Merck Millipore columns are designed to fit any HPLC instrument,; however, careshould be taken at installation so as not to introduce dead volumes in the connections, which would reduce separation efficiency. Note that stainless steel tubing fitting are inflexible and cannot beadapted to different port designs after the first installation, whereas PEEK fittings can be adjusted for different columns several times. Also note that stainless steel fittings and ferrules can damagethe end fittings of PEEK column hardware, especially if installed with excessive force by using wrench tools.
Merck Millipore columns should be installed with the flow arrow on label pointing towardsthe detector. Before the column outler is connected to the detector, it is wise to flush the column with mobile phase.




EQUILIBRADO DE LA COLUMNA
Equilibrio adecuado columna es un tiempo bienempleado, ya que le dará resultado más consistente y reducir la resolución de problemas. Esto es de especial importancia con cartuchos LiChroCART, debido a que las columnas no son cerradas y sepueden secar fácilmente. Cartuchos LiChroCART necesitan ser vaciados correctamente antes de su uso.
Compruebe que la fase móvil es miscible con el disolvente de envío antes de comenzar a limpiar oequilibrar la columna.

INSTALACIÓN COLUMNA
Merck Millipore columnas están diseñados para adaptarse a cualquier instrumento HPLC,; Sin embargo, se debe tener cuidado en la instalación a fin de no...