Aeromonas bestiarum

Notas de investigación
Aislamiento e identificación de Aeromonas bestiarum a partir de carpa común de cultivo (Cyprinus carpio L.) procedentes de Santa María Chapa de Mota, Estado de México, México Isolation and identification of Aeromonas bestiarum in cultured common carp (Cyprinus carpio L.) from Santa Maria Chapa de Mota, Estado de Mexico, Mexico
Edgardo Soriano-Vargas* GracielaCastro-Escarpulli** Ma. Guadalupe Aguilera-Arreola** Fernando Vega-Castillo* Celene Salgado-Miranda*

Abstract
The isolation of Aeromonas bestiarum from common carps (Cyprinus carpio L.), cultivated at Santa Maria Chapa de Mota, is reported for the first time. The genetic identification for differentiating A. bestiarum from A. salmonicida is here emphasized.

Key words:AEROMONAS BESTIARUM, CARP,AQUACULTURE, MEXICO.

Resumen
Se informa por primera ocasión el aislamiento de Aeromonas bestiarum a partir de carpa común (Cyprinus carpio L.) de cultivo, procedente de Santa María Chapa de Mota, Estado de México, México. Se enfatiza la identificación genética para la diferenciación entre A. salmonicida y A. bestiarum.

Palabras clave: AEROMONAS BESTIARUM, CARPA, ACUACULTURA, MÉXICO.

Recibido el3 de septiembre de 2009 y aceptado el 26 de marzo de 2010. *Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Carretera Panamericana Toluca-Atlacomulco, km 15.5, Toluca, 50200, México, Tel./Fax: (722) 2965555, correo electrónico: soriano@uaemex.mx **Laboratorio de Bacteriología Médica, EscuelaNacional de Ciencias Biológicas, Instituto Politécnico Nacional. Plan de Ayala y Carpio s/n, México, D. F., 11340, Tel. 57296300 ext. 62374.

Vet. Méx., 41 (2) 2010

111

Introduction

T

Introducción

he members of the genus Aeromonas are gramnegative rods, inhabitants of a wide variety of environments, although freshwater-associated aquatic fauna and sediments are the most frequenthabitats. Infection and disease in several animal species, fish and humans are caused by some Aeromonas species.1 Diagnosis is based on the bacteriological isolation and biochemical identification of the agent. Particularly, salmonid furunculosis is mainly caused by A. salmonicida, while A. bestiarum has been isolated from diseased fish and its pathogenicity to common carp demonstrated.2 A.salmonicida strains are non-motile and cause hemolysis. Five subspecies are currently recognized: A. salmonicida subspecies achromogenes, A. salmonicida subspecies masoucida, A. salmonicida subspecies pectinolytica, A. salmonicida subspecies smithia and A. salmonicida subspecies salmonicida. Many laboratories classify A. salmonicida subspecies salmonicida as “typical” and any isolate with phenotypicdifferences as “atypical”.3 Strains of A. bestiarum are usually hemolytic and motile.2* However, an accurate identification between A. salmonicida and A. bestiarum may be limited by differences in phenotypic characteristics of the isolates. In Mexico, another mesophylic, motile Aeromonas species (i. e., A. hydrophila) have been isolated from several cultured fish destined to human consumption andornamental such as rainbow trout (Oncorhynchus mykiss),4,5 tilapia (Oreochromis aureus; O. niloticus niloticus),4,6 goldfish (Carassius auratus auratus)7 and charal (Chirostoma humboldtianum).8 The isolation of A. bestiarum from cultured common carps (Cyprinus carpio L.) from Santa Maria Chapa de Mota, Estado de Mexico, Mexico, is reported for the first time in the present work. As part of theepidemiological surveillance program carried out by the Comite de Sanidad Acuicola del Estado de Mexico, A. C. (CSAEM), cultured common carps sourced from a pond located in Santa Maria Chapa de Mota, were submitted for routine diagnosis. This is a multiage (fingerling, juvenile and adult) farm containing 3 000 fish in semi-intensive ponds. Fish feed on plankton and coexist with wild frogs. The farmer did...