Aislamiento De Eps

A novel exopolysaccharide from probiotic Lactobacillus fermentum CFR 2195: Production, purification and characterization
Vimlesh Yadav1, Siddalingaiya Gurudutt Prappulla1, Alok Jha2, Amrita Poonia21

Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore, Karnataka 570020, India; Email: vimy249@gmail.com 2 Centre of Food Science andTechnology, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India

ABSTRACT
Exopolysaccharides (EPS) from Lactic Acid Bacteria (LAB) contribute to specific rheology and texture of novel foodproducts and finds applications even in therapeutics. The structure and amount of EPS produced by LAB varies with the strains and cultural conditions. The human faecal matter isolate Lactobacillusfermentum CFR 2195 was found to produce an exopolysaccharide in quantitative amounts. The objective of the present study was the production, purification and characterization of exopolysaccharide fromLactobacillus fermentum CFR 2195. The EPS was produced in simplified synthetic medium and extracted with two volumes of ethanol after 24 h of fermentation. The quantity of EPS produced by isolate was28.82 g/L. The dialyzed and lyophilized EPS was used in Scanning Electron Microscopy (SEM), High Performance Liquid Chromatography (HPLC) and Fourier Transform Infrared Spectroscopy (FTIR) formorphological, chemical and compositional characterization, respectively. The EPS was found highly compact and flakes like structures by SEM micrograph. The HPLC chromatogram of EPS indicates that the EPS isheteroploysaccharide consisting of rhamnose (94%), raffinose (3.6%) and maltose (2.5%). Based on the FTIR spectrum major functional group of EPS was found to be Carbonyl (C=O), Hydrocarbon (C-H),Carboxylic (-COOH), Amine (-NH2) and aromatic ring (-C6H6), which indicate chemical complexity of the EPS. Hence, the study concludes that the EPS of Lactobacillus fermentum CFR 2195 is highly compact,...