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Methods in Parasitology

8. KATO-Katz technique for helminth eggs

Swiss Tropical Institute, Basel April 2005

8.0 KATO-Katz technique for helminth eggs

Contents • 8.1 Materials • 8.2Procedure

Production Team: Yvette Endriss, Elisabeth Escher & Birgit Rohr Prof. Hanspeter Rohr (NeoCortex Foundation) Prof. Niklaus Weiss (STI)

8.1 KATO-Katz technique for helminth eggs
Materials:• Kato-set (Template with hole, screen, nylon or plastic, plastic spatula) • Newspaper or glazed tile • Microscope slides • Cellophane as cover slip, soaked in Glycerol-malachite green solution •Fresh stool • Gloves

8.2 KATO-Katz technique for helminth eggs

Prepare the layer Glazed tile or newspaper

Place the template with hole in the centre of a microscope slide

8.3 KATO-Katztechnique for helminth eggs

Use gloves !

Place a small amount of faecal material on the newspaper or the glazed tile.

8.4 KATO-Katz technique for helminth eggs
Press the screen on top so thatsome of the faeces filters through and scrape with the flat spatula across the upper surface to collect the filtered faeces.

Add the collected faeces in the hole of the template so that it iscompletely filled.

8.5 KATO-Katz technique for helminth eggs

Remove the template carefully so that the cylinder of faeces is left on the slide.

Cover the faecal material with the pre-soakedcellophane strip.

8.6 KATO-Katz technique for helminth eggs
Invert the microscope slide and firmly press the faecal sample against the cellophane strip on a smooth hard surface such as a tile. Thematerial will be spread evenly. Carefully remove the slide by gently sliding it sideways to avoid separating the cellophane strip. Place the slide with the cellophane upwards.

8.7 KATO-Katztechnique for helminth eggs

The smear should be examined in a systematic manner and the eggs of each species reported. Later multiply by the appropriate number (see inlet-information of the Kato-set) to...