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Determination of Extractives in Biomass
Laboratory Analytical Procedure (LAP) Issue Date: 7/17/2005
A. Sluiter, R. Ruiz, C. Scarlata, J. Sluiter, and D. Templeton

Technical Report
NREL/TP-510-42619 January 2008NREL is operated by Midwest Research Institute ● Battelle

Contract No. DE-AC36-99-GO10337

Determination of Extractives in Biomass
Laboratory Analytical Procedure (LAP) Issue Date: 7/17/2005
A. Sluiter, R. Ruiz, C. Scarlata, J. Sluiter, and D. Templeton

Technical Report
NREL/TP-510-42619 January 2008

NREL Laboratory Analytical Procedures for standard biomass analysis are availableelectronically at http://www.nrel.gov/biomass/analytical_procedures.html

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Procedure Title: Determination of Extractives in Biomass
Laboratory Analytical Procedure
1. Introduction 1.1 It is necessary to remove non-structural material from biomass prior to analysis to prevent interference with later analytical steps. This procedure uses a two-step extraction process to remove water soluble and ethanol soluble material.Water soluble materials may include inorganic material, non-structural sugars, and nitrogenous material, among others. Inorganic material in the water soluble material may come from both the biomass and any soluble material that it is associated with the biomass, such as soil or fertilizer. No attempt is made to distinguish the source of the inorganic material. Ethanol soluble material includeschlorophyll, waxes, or other minor components. Some biomass may require both extraction steps, while other biomass may only require exhaustive ethanol extraction. 1.2 Refer to LAP-010 or ASTM Standard Test Method E 1690 “Determination of Ethanol Extractives in Biomass” for extraction procedures for isolation and characterization of extractives. 1.3 This procedure covers the determination of soluble...