Bacteria Con Genoma Sintetizado Quimicamente

Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome Daniel G. Gibson et al. Science 329, 52 (2010); DOI: 10.1126/science.1190719

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RESEARCH ARTICLE Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Daniel G. Gibson,1 John I. Glass,1 Carole Lartigue,1 Vladimir N. Noskov,1 Ray-Yuan Chuang,1 Mikkel A. Algire,1 Gwynedd A. Benders,2 Michael G. Montague,1 Li Ma,1 Monzia M. Moodie,1 Chuck Merryman,1 Sanjay Vashee,1 Radha Krishnakumar,1 NacyraAssad-Garcia,1 Cynthia Andrews-Pfannkoch,1 Evgeniya A. Denisova,1 Lei Young,1 Zhi-Qing Qi,1 Thomas H. Segall-Shapiro,1 Christopher H. Calvey,1 Prashanth P. Parmar,1 Clyde A. Hutchison III,2 Hamilton O. Smith,2 J. Craig Venter1,2* We report the design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and itstransplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable ofcontinuous self-replication. n 1977, Sanger and colleagues determined the complete genetic sequence of phage ϕX174 (1), the first DNA genome to be completely sequenced. Eighteen years later, in 1995, our team was able to read the first complete genetic sequence of a self-replicating bacterium, Haemophilus influenzae (2). Reading the genetic sequence of a wide range of species has increasedexponentially from these early studies. The ability to rapidly digitize genomic information has increased by more than eight orders of magnitude over the past 25 years (3). Efforts to understand all this new genomic information have spawned numerous new computational and experimental paradigms, yet our genomic knowledge remains very limited. No single cellular system has all of its genes understood in...