Bioquimica

The Laboratory Biosafety Guidelines
3rd Edition

Published by authority of the Minister of Health Population and Public Health Branch Centre for Emergency Preparedness and Response

Our mission is to help the people of Canada maintain and improve their health. Health Canada

Également disponible en français sous le titre Lignes directrices en matière de biosécurité en laboratoire

Allrights reserved. No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, without prior written permission of the Minister of Public Works and Government Services, Ottawa, Ontario, K1A 0S5

Cover photographs firstlight.ca; premiumstock/firstlight.ca; PascalParrot/corbis/firstlight.ca

© Her Majesty the Queen in Right of Canada, represented by the Minister of Health (2004) Cat. N° H39-4/49-2004E H39-4/49-2004E-PDF H39-4/49-2004E-HTML ISBN 0-662-37722-2 0-662-37723-0 0-662-37724-9 Publication N° 4252

Editorial Committee
Maureen Best Director Office of Laboratory Security Health Canada Ottawa, ON Mary Louise Graham Chief, Biosafety Division Office ofLaboratory Security Health Canada Ottawa, ON Roland Leitner Environmental Safety Officer University of Calgary Calgary, AB Marc Ouellette Professeur, Centre hospitalier de l’université Laval Québec, QC Ken Ugwu Senior Biocontainment Engineer Office of Laboratory Security Health Canada Ottawa, ON

James Campbell Chair, University Biosafety Committee University of Toronto Toronto, ON Wayne Conlan ResearchOfficer National Research Council Ottawa, ON Pamela Dunn Research Scientist Aventis Pasteur Ltd. North York, ON Sandra Fry Director, Biohazard Containment and Safety Division, Canadian Food Inspection Agency Ottawa, ON Fred George (Large Scale Production) Alberta Research Council Edmonton, AB Andrew Graham (Large Scale Production) Aventis Pasteur Limited Toronto, ON David Groves Service ofMedical Microbiology St. Joseph’s Hospital Professor, Dept. of Pathology and Molecular Medicine Faculty of Health Sciences McMaster University Hamilton, ON Toni Hays (Large Scale Production) Apotex Fermentation Inc. Winnipeg, MB

Contributors
The development of the 3rd edition of the Laboratory Biosafety Guidelines has been made possible thanks to the contributions of the following individuals:Harvey Artsob Chief, National Laboratory for Zoonotic Diseases and Special Pathogens Canadian Science Centre for Human & Animal Health Winnipeg, MB

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Marianne Heisz Chief, Bioterrorism and Emergency Response Division Office of Laboratory Security Health Canada Ottawa, ON Jean Joly Directeur, Laboratoire de santé publique du Québec Ste-Anne-de-Bellevue, QC George Khachatourians (Large ScaleProduction) Department of Applied Microbiology and Food Science University of Saskatchewan Saskatoon, SK Paul Langevin (Technical Advisor) Biocontainment Services Kanata, ON Louise Linarez Technical Supervisor Provincial Laboratory of Public Health Edmonton, AB Jim Orzechowski (Technical Advisor) Chief Executive Officer Smith Carter Architects and Engineers Inc. Winnipeg, MB Mark Parrington PrincipalResearch Scientist and Section Head Microbiology Aventis Pasteur Limited North York, ON

Bruce Peat (Technical Advisor) General Manager H.E.P.A. Filter Services Inc. Concord, ON Anthony Ridgway (Large Scale Production) Therapeutic Products Program Health Canada Ottawa, ON Richard Stokes Associate Professor University of British Columbia Vancouver, BC Lee Thompson Director of Biosafety andInstitutional Containment Resources The University of Texas Medical Branch Galveston, TX, U.S.A. Stefan Wagener Scientific Director Office of Biosafety and Environment Canadian Science Centre for Human and Animal Health Winnipeg, MB

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Acknowledgements
We would like to acknowledge and thank Danielle Plouffe and Jasmine Boudreau for their clerical assistance in the preparation of this document,...