Cereales

Journal of Cereal Science 48 (2008) 258e276
www.elsevier.com/locate/jcs

Review

Is the in vitro antioxidant potential of whole-grain
cereals and cereal products well reflected in vivo?
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Anthony Fardet*, Edmond Rock, Christian Remesy
Unite de Nutrition Humaine, INRA-UMR 1019, Centre de Recherche de Clermont-Ferrand/Theix, 63122 Saint-Genes-Champanelle, France
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Received 14 November2007; received in revised form 22 January 2008; accepted 23 January 2008

Abstract
There is strong epidemiological evidence that whole-grain cereals protect the body against age-related diseases such as diabetes, cardiovascular diseases and some cancers. This may be due to the fibre and micronutrients in the outer layer and germ fractions of the grain acting together
to combat oxidativestress, inflammation, hyperglycaemia and carcinogenesis. Oxidative stress is associated with these metabolic diseases.
Whole-grain cereals are a good source of vitamin E, folates, phenolic acids, zinc, iron, selenium, copper, manganese, carotenoids, phytic
acid, lignins, lignans, and alkylresorcinols, all of which have significant antioxidant potential in vitro. Phenolic acids such as ferulic acid arecharacteristic of cereals. They may scavenge free-radical oxygen species both in vitro and in vivo. Phenolics may also act in vivo by triggering
gene induction/repression via cell signalling through transcription factors. Whole-grain cereals are also a good source of betaine, choline and
sulphur amino acids that can improve antioxidant status. Betaine, which accounts for about 1% (w/w) of thebran fraction in wheat, acts as
a methyl donor that may decrease hyperhomocysteinaemia, a cardiovascular risk factor, but it has been neglected. Cereals and cereal products
are antioxidative in vitro, as are most fruits and vegetables. The in vitro antioxidant capacity of cereals and their constituent fractions is significantly correlated with their polyphenol content, except for maize. However,the in vitro antioxidant capacity of cereals is only an approximate
reflection of their in vivo antioxidant effect due to differences in antioxidant solubility/bioavailability within the digestive tract and the metabolism/conjugation of compounds such as polyphenols. During digestion, the antioxidant capacity of cereals is increased and is likely to provide
a favourable antioxidative environmentfor the epithelium tract, notably in the large intestine. Most of the studies performed on animals have
been concerned with the antioxidant property of coloured rice, especially black rice and its anthocyanin fraction, showing a positive effect on
some antioxidant biomarkers. Those very few studies that have been done on humans have shown that wheat bran and rye product supplements
have noeffect on antioxidant status, while a black rice pigment fraction and an avenanthramide-enriched mixture extracted from hulled oats have
a positive effect. In vivo studies are therefore needed to further explore the real antioxidant potential of cereals.
Ó 2008 Elsevier Ltd. All rights reserved.
Keywords: Whole-grain cereals; Micronutrients; In vitro Antioxidant potential; In vivo studiesContents
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Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Whole-grain cereals as a source of antioxidants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.1. Vitamin E . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.2. Folates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ....