Dengue

International Clinical Evaluation Reports

Keep challenging diagnosis for Dengue
SD BIOLINE Dengue Duo(NS1 Ag + Ab Combo) & ELISA

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Contents
1. Tricou et al. BMC Infectious Diseases 2010, 10:142
Comparison of two dengue NS1 rapid tests for sensitivity, specificity and relationship to viraemia and antibody responses Oxford University ClinicalResearch Unit, Hospital for Tropical Diseases, Ho Chi Minh City PERFORMANCE / Sensitivity 83.7% & Specificity 97.9%

2. Am. J. Trop. Med. Hyg. 83(3), 2010, pp. 690–695
Early Diagnosis of Dengue Infection Using a Commercial Dengue Duo Rapid Test Kit for the Detection of NS1, IGM, and IGG Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Malaysia PERFORMANCE /Sensitivity 88.65% & Specificity 98.75%

3. JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 2010
Evaluation of a Commercial SD Dengue Virus NS1 antigen Capture Enzyme-Linked Immunosorbent Assay Kit for Early Diagnosis of Dengue Virus Infection Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Malaysia PERFORMANCE / Sensitivity 76.76% & Specificity 98.31%

4. Inform’ACTION Aug, 2010/ Pacific Public Health Surveillance Network (PPHSN)
Assessment of NS 1 antigen detection tests during DEN -4 epidemic in French Polynesia Institut Louis Malarde Papeete, French Polynesia PERFORMANCE / Sensitivity 76.5% & Specificity 96.2%

Tricou et al. BMC Infectious Diseases 2010, 10:142 http://www.biomedcentral.com/1471-2334/10/142

RESEARCH ARTICLE
Research article

Open AccessComparison of two dengue NS1 rapid tests for sensitivity, specificity and relationship to viraemia and antibody responses
Vianney Tricou*1, Hang TT Vu1, Nhu VN Quynh1, Chau VV Nguyen2, Hien T Tran2, Jeremy Farrar1,3, Bridget Wills1,3 and Cameron P Simmons1,3

Abstract Background: Dengue is a major public health problem in tropical and subtropical countries. Rapid and easy diagnosis of dengue canassist patient triage and care-management. The detection of DENV NS1 on rapid lateral flow tests offers a fast route to a presumptive dengue diagnosis but careful evaluations are urgently needed as more and more people use them. Methods: The sensitivity and specificity of the Bio-Rad NS1 Ag Strip and SD Dengue Duo (NS1/IgM/IgG) lateral flow rapid tests were evaluated in a panel of plasma samplesfrom 245 Vietnamese patients with RT-PCR confirmed dengue and 47 with other febrile illnesses. Results: The NS1 rapid tests had similar diagnostic sensitivities (respectively 61.6% and 62.4%) in confirmed dengue cases but were 100% specific. When IgM/IgG results from the SD Dengue Duo were included in the test interpretation, the sensitivity improved significantly from 62.4% with NS1 alone to75.5% when NS1 and/or IgM was positive and 83.7% when NS1 and/or IgM and/or IgG was positive. Both NS1 assays were significantly more sensitive for primary than secondary dengue. NS1 positivity was associated with the underlying viraemia as NS1-positive samples had a significantly higher viraemia than NS1-negative samples. Conclusions: These data suggest that the NS1 test component of these assays arehighly specific and have similar levels of sensitivity. The IgM parameter in the SD Duo test improved overall test sensitivity without compromising specificity. The SD Dengue Duo lateral flow rapid test deserves further prospective evaluation in dengue endemic settings. Background Dengue is a major public health problem in tropical and subtropical countries [1,2]. Each of the four serotypes ofdengue virus (DENV-1 to DENV-4) is able to cause disease. Clinically apparent disease varies in severity from mild undifferentiated fever through to more severe syndromes, such as dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). DHF is a vasculopathy characterized by capillary leakage and haematological deregulation, which in severe cases can result in lifethreatening hypovolemic...