Elisa Anti-Dna

Product details for DNA (Double Stranded) ELISA Kit
Please address orders & technical inquiries to:
antibodies-online GmbH, Schloß-Rahe-Str. 15, D-52074 Aachen
Ordering via fax +49 241 95 163 155 or via phone +49 241 95 163 153
support@antibodies-online.com
Catalog no.: ABIN364954

Overview
Type

Kit

Antigen

DNA (Double Stranded)

Sample Type

Serum, Plasma

ApplicationELISA

Quantity

96 tests

Catalog no.

ABIN364954

Additional Information
Characteristics

This immunoassay kit allows for the in vitro semi-quantitative determination of human 
dsDNA (IgG) concentrations in serum, plasma and other biological fluids.

Antigen

DNA (Double Stranded)

Alternative name

Double stranded DNA (dsDNA IgG)

Reactivity

Human

Sample TypeSerum, Plasma

Specificity

This assay recognizes human dsDNA (IgG). No significant cross-reactivity or interference 
was observed.

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Application Details
Principle

The microtiter plate provided in this kit has been pre-coated with purifieddsDNA-BSA. 
Samples are then added to the appropriate microtiter plate wells and incubated. Then add 
Horseradish Peroxidase (HRP)-conjugated anti-human IgG to each well and incubate. Finally, 
a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The 
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and 
the color change ismeasured spectrophotometrically at a wavelength of 450 nm ± 2 nm. 
Calculate the valence of dsDNA (IgG) in the samples.

Protocol

Preparation of Reagents: Bring all reagents to room temperature before use. 1. Wash Buffer 
If crystals have formed in the concentrate, warm up to room temperature and mix gently 
until the crystals have completely dissolved. Dilute 20 ml of Wash Buffer Concentrateinto 
deionized or distilled water to prepare 500 ml of Wash Buffer. 2. HRP-anti-human IgG 
Dilute to the working concentration specified on the vial label using HRP-anti-human IgG 
Diluent(1:100), respectively. Precaution: The Stop Solution provided with this kit is an 
acid solution. Wear eye, hand, face, and clothing protection when using this material. 
Sample Collection and Storage: CellCulture Supernates Remove particulates by 
centrifugation and assay immediately or aliquot and store samples at -20° C. Avoid 
repeated freeze-thaw cycles. Serum Use a serum separator tube (SST) and allow samples to 
clot for 30 minutes before centrifugation for 15 minutes at 1000 g. Remove serum and assay 
immediately or aliquot and store samples at -20° C. Avoid repeated freeze-thaw cycles. Plasma Collect plasma using citrate, EDTA, or heparin as an anticoagulant. Centrifuge for 
15 minutes at 1000 g within 30 minutes of collection. Assay immediately or aliquot and 
store samples at -20° C. Avoid repeated freeze-thaw cycles. Assay Procedure: Calculation 
of Results: For calculation the valence of human dsDNA(IgG), please dilute the sample from 
1:40 using Sample Diluent. Comparethe sample well with control. The dilution factor with 
significant difference color is determined as the final valence. Limitations of the 
Procedure: The kit should not be used beyond the expiration date on the kit label. Do not 
mix or substitute reagents with those from other lots or sources. Any variation in 
operator, pipetting technique, washing technique, incubation time ortemperature, and kit 
age can cause variation in binding. This assay is designed to eliminate interference by 
soluble receptors, binding proteins, and other factors present in biological samples. 
Until all factors have been tested in the Immunoassay, the possibility of interference 
cannot be excluded.

Prot. Assay Procedure

Assay Time: 1-3hSample Volume: 50-100ul

Application

ELISA...