Ensayo de fagocitosis macrófagos thp1 a celulas tumorales a427

24/04/2009 Ensayo de fagocitosis Macrófagos THP1 a celulas tumorales A427

Blanco
Multiple Document Interface for Flow Cytometry Multiple Document Interface for Flow Cytometry
WinMDI Version 2.8 - Windows 3.95/DOS 5.0 WinMDI Version 2.8 - Windows 3.95/DOS 5.0
Fri May 08 13:26:44 2009Fri May 08 13:27:40 2009
Gates: Gates: R1*R2
Project: Experiment: Project: Experiment:
File: THP1.001 Sample: File: THP1.001 Sample:
Date: 27-Apr-09 Parameters: 7 Date: 27-Apr-09 Parameters: 7
Total Events 14175 Gated Events 14175 100.00% Total Events 14175 Gated Events 11199 79.01%
System:Log Parameter Means: Arithmetic System: Log Parameter Means: Arithmetic
TAMANO (1) vs GRANULARIDAD (2) Quad Stats
Region Events %Total %Gated PKH26(Log) vs CD45 PE Cy5(Log)
R0 14175 100 100 Quadrant x,y: 260,232
R1 11827 83.44 83.44 Quad Events %Total %Gated
R2 12121 85.51 85.51 1UL 21 0.15 0.19
2 UR 123 0.87 1.1
3 LL 10878 76.74 97.13
4 LR 177 1.25 1.58



THP1 aCD45 Cy5
Multiple Document Interface for Flow Cytometry Multiple Document Interface for Flow Cytometry
WinMDI Version 2.8 - Windows 3.95/DOS 5.0WinMDI Version 2.8 - Windows 3.95/DOS 5.0
Fri May 08 13:59:38 2009 Fri May 08 14:03:07 2009
Gates: Gates: R1*R2
Project: Experiment: Project: Experiment:
File: THP1.003 Sample: File: THP1.003 Sample:
Date: 27-Apr-09 Parameters: 7 Date: 27-Apr-09 Parameters: 7
Total Events 26353 Gated Events 26353100.00% Total Events 26353 Gated Events 20844 79.10%
System: Log Parameter Means: Arithmetic System: Log Parameter Means: Arithmetic
TAMANO (1) vs GRANULARIDAD (2) Quad Stats
Region Events %Total %Gated PKH26(Log) vs CD45 PE Cy5(Log)
R0 26353 100 100 Quadrant x,y: 268,288
R1 22234 84.37 84.37Quad Events %Total %Gated
R2 22511 85.42 85.42 1 UL 20400 77.41 97.87
R3 25630 97.26 97.26 2 UR 109 0.41 0.52
3 LL 332 1.26 1.59
4 LR 3 0.01 0.01




A427 PKH26
Multiple Document Interface for Flow CytometryMultiple Document Interface for Flow Cytometry
WinMDI Version 2.8 - Windows 3.95/DOS 5.0 WinMDI Version 2.8 - Windows 3.95/DOS 5.0
Fri May 08 13:41:29 2009 Fri May 08 13:41:57 2009
Gates: Gates: R1*R2
Project: Experiment: Project: Experiment:
File: THP1.002 Sample: File: THP1.002 Sample:Date: 27-Apr-09 Parameters: 7 Date: 27-Apr-09 Parameters: 7
Total Events 5070 Gated Events 5070 100.00% Total Events 5070 Gated Events 1820 35.90%
System: Log Parameter Means: Arithmetic System: Log Parameter Means: Arithmetic
TAMANO (1) vs GRANULARIDAD (2) Quad Stats
Region Events %Total %GatedPKH26(Log) vs CD45 PE Cy5(Log)
R0 5070 100 100 Quadrant x,y: 268,288
R1 2757 54.38 54.38 Quad Events %Total %Gated
R2 2954 58.26 58.26 1 UL 0 0 0
R3 1640 32.35 32.35 2 UR 77 1.52 4.23
3 LL 138 2.72 7.58
4 LR 1605 31.66 88.19


C- THP1/A427...