enzima

have you had a blood transfusion
have you had reaction to the blood transfusion
have you had alergia
when were you born.
Do you have a doctor in this hospital
what her name.
DOLOR
Do youhave pain
where is the pain
what kind of pain is it: sharp ( agudo),fixed ( fijo) burning (quemadura).
when did you start the pain
have you had a blood transfusion
have you had reaction to theblood transfusion
have you had alergia
when were you born.
Do you have a doctor in this hospital
what her name.
DOLOR
Do you have pain
where is the pain
what kind of pain is it: sharp (agudo),fixed ( fijo) burning (quemadura).
when did you start the pain
have you had a blood transfusion
have you had reaction to the blood transfusion
have you had alergia
when were you born.
Doyou have a doctor in this hospital
what her name.
DOLOR
Do you have pain
where is the pain
what kind of pain is it: sharp ( agudo),fixed ( fijo) burning (quemadura).
when did you start thepain
EXTRACCIÓN DEL DNA
(20 puntos)
OBJETIVO
Separar el material genético de sus otros componentes celulares y visualizar el ADN como un sobrenadante color blanco y pegajoso.
MATERIALES
fruta ovegetal (fresas, kiwi, tomates, lechuga)
licuadora (osterizer)
gaza o colador
embudo (1)
taza de medir 8 onzas (1)
cucharas de medir (1 juego)
estufa
vasos pequeños de cristal transparente (5)100 ml solución amortiguadora:
10 ml detergente**
10 g sal
90 ml agua
**puede ser líquido de fregar, champú, detergente de lavar ropa líquido.
100 ml alcohol etílico, alcohol isopropílico 70%"Rubbing alcohol"
PROCEDIMIENTO
1. Haga un licuado de la fruta añadiéndole agua. El licuado separa las células. No le debe añadir mucha agua, pues no habrá suficiente DNA visible.
2. Coloque el licuadoen la gaza y ponga en un vaso con un embudo. (ver foto 1)
3. Añada 50 ml de la solución amortiguadora al licuado y espere 10 minutos.
4. Filtre directamente de la gasa al vaso con la ayuda del...