genoma



UNIVERSIDAD DE GUAYAQUIL
FACULTAD DE CIENCIAS MÉDICAS
ESCUELA DE OBSTETRICIA



TEMA:

PAPER: “Células Stem”.



CÁTEDRA:

Biología Molecular y Celular


CATEDRÁTICO:

Dr.Nancibel Manrique Bojórquez


FECHA:

08/11/2013

Human placenta-derived mesenchymal stem cells acquire neural phenotype under the appropriate niche conditions.

Martini MM, Jeremias Tda S,Kohler MC, Marostica LL, Trentin AG, Alvarez-Silva M.

Source
Laboratório de Células Tronco e Regeneração Tecidual, Departamento de Biologia Celular, Embriologia e Genética, Universidade Federal deSanta Catarina, Campus Universitario, Florianópolis, SC, Brazil.

Abstract
Mesenchymal stem cells (MSCs) are multipotent stem cells with clinical interest. It has been reported that MSCs can beisolated from the human term placenta. We investigated the ability of human placenta-derived MSCs to differentiate into a neural phenotype in coculture assays with astrocytes obtained from neonatal rats.Placenta-derived MSCs were cocultured on a confluent monolayer of astrocytes obtained from the rat cerebellum to evaluate the differences in morphology. The extracellular matrix (ECM) produced byastrocytes as well as the growth factors produced by the astrocyte-conditioned medium were evaluated. The expression of the neural markers glial fibrillate acid protein (GFAP) and Nestin was studied in MSCsby immunocytochemistry. MSCs were able to respond to the astrocyte niche in coculture assays. They expressed the neural markers GFAP, Nestin, or β-Tubulin III, followed by an outgrowth of cellprocesses. The ECM from astrocytes was not effective in inducing the neural phenotype in MSCs, although the expression of β-Tubulin III was observed. When MSCs were cocultured with cerebellar astrocytesfrom newborn rats, a neural phenotype was achieved. This was determined by immunocytochemistry to GFAP, Nestin, or β-Tubulin III and by morphological changes. It was achieved without the addition of...