Glutation peroxidasa

Int Arch Occup Environ Health DOI 10.1007/s00420-008-0378-1

O R I G I N A L A R T I CL E

Cytological damage of nasal epithelium associated with decreased glutathione peroxidase in residentsfrom a heavily polluted city
S. A. Hernández-Escobar · M. C. Ávila-Casado · V. Soto-Abraham · O. L. López Escudero · M. E. Soto · M. L. Vega-Bravo · T. Fortoul van der Goes · Elba Reyes-MaldonadoReceived: 18 December 2007 / Accepted: 21 October 2008 © Springer-Verlag 2008

Abstract Objective To evaluate the cytological damage and glutathione peroxidase (GPX) content in the nasal epithelium ofresidents of Southwest Metropolitan Mexico City (SWMMC) along 1 year of ozone and PM10 exposure. Method Four nasal scrapings were obtained in 20 volunteers from a control low polluted city and SWMMCpermanent residents (n = 20) during 1 year. The scrapings were obtained in September and December 2004, and February and May 2005. One part of the scraping was stained by hematoxylin–eosin techniquefor cytological evaluation and a second part was stained by immunocytochemistry method to evaluate GPX concentration by morphometry.

S. A. Hernández-Escobar · M. C. Ávila-Casado · V. Soto-Abraham ·M. E. Soto Pathology Department, Instituto Nacional de Cardiología Ignacio Chávez, Mexico, Mexico S. A. Hernández-Escobar · M. L. Vega-Bravo · E. Reyes-Maldonado Cytology Laboratory, MorphologyDepartment, Instituto Politécnico Nacional Prol. de Carpio y Plan de Ayala, 11340 Mexico, Mexico T. F. van der Goes Cellular and Tissue Biology Department, Facultad de Medicina, Universidad Nacional Autónomade México, Mexico, Mexico O. L. López Escudero Instituto Latinoamericano de Comunicación Educativa, Mexico, Mexico E. Reyes-Maldonado (&) Av. Centenario 419 Int A-103 Col. Nextengo, Azcapotzalco D.F,C.P. 02070, Mexico, Mexico e-mail: relba@hotmail.com

Results Control subjects: in total, 30% had no cytological alterations and 70% showed only mild or moderate inXammation in four nasal...