Gnes en botella

Biotechnology Explorer™
Genes in a Bottle Kit DNA Extraction Module
Catalog Number 166-2000EDU DNA necklace module (166-2200EDU) must be purchased separately. explorer.bio-rad.com
See individual components for storage temperature.

Duplication of any part of this document is permitted for classroom use only.

For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call1-800-4BIORAD (1-800-424-6723)

Capture Your Essence!
Bottle your DNA! Whether it’s being cloned, sequenced, fingerprinted, mapped, or genetically engineered, DNA has become an everyday topic in the media and the classroom. Introduce your students to the molecular framework of biology — with their own DNA! How do scientists separate pure DNA from cells composed mainly of lipids,proteins, carbohydrates, and salts? Membranes are first ruptured with detergents to release DNA into a solution; then proteins and other organic molecules are digested and separated while retaining intact DNA. The DNA is finally collected by precipitation in a form that can be manipulated as desired. With this simple lab activity, students gain practical knowledge by conducting a real-worldprocedure that is used to extract DNA from many different organisms for a variety of applications. Your students will extract genomic DNA from their own cheek cells and watch it precipitate from solution as floating white strands. The DNA strands are then easily collected and transferred to a glass vial, and the vial is fashioned into a necklace! Seeing is believing. For students learning aboutthe molecular framework of biology for the first time, DNA is abstract and intangible. This procedure makes the invisible visible — seeing their own DNA makes it real and helps students comprehend this previously invisible substance of life. Learning opportunities for all levels of instruction. This activity is designed for any classroom environment and requires no specialized equipment orstains. For secondary and college level instruction, lessons on DNA structure and function, cell structure, and enzyme function can be introduced or reinforced with this laboratory activity. For middle school students, it’s a perfect introduction to the exciting world of DNA science. We welcome your comments and suggestions. Have fun! Ingrid Hermanson-Miller, Ph.D. Biotechnology ExplorerProduct Manager Melissa Woodrow, Ph.D. Biotechnology Explorer Scientist

Create context. Reinforce learning. Stay current. New scientific discoveries and technoligies create more content for you to teach, but not more time. Biotechnology Explorer kits help you teach more effectively by integrating multiple core content subjects into a single lab. Connect concepts with techniques and put theminto context with real-world scenarios.

• Conduct sophisticated scientific procedures • Extract DNA from cheek cells • Precipitate and preserve DNA

Environmental and Health Science
• Genetic testing • DNA fingerprinting

Scientific Inquiry

Genes in a Bottle Kit
• Cell structures • Organelles • Nuclear and DNA staining • Cell organization

Chemistry of Life
• Chemicalproperties of cell components • Properties of enzymes • Solubility

Structure and Function of Organisms

Heredity and Molecular Biology

• DNA and genetic variation among individuals • Genes are inherited

Evolutionary Biology

• Central dogma: DNA > RNA > protein > trait • DNA location, structure, and function • Basic review of chromosome inheritance and structure

Table of ContentsTeacher’s Guide
Kit Inventory Checklist ..................................................................................................1 Overview for the Teacher ..............................................................................................2 Why Shoud You Teach DNA Extraction? ..................................................................2 Intended Audience...