Inos2
Páginas: 4 (815 palabras)
Publicado: 11 de octubre de 2011
SANTA CRUZ BIOTECHNOLOGY, INC.
NOS2 (N-20): sc-651
BACKGROUND
Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogeneticallydiverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requireshomodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have beendescribed and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including ncNOS (also designated NOS1) whose activity was firstidentified in neurons and maps at 12q24.2, and ecNOS (also designated NOS3), first identified in endothelial cells and mapping at 7q35-36. The inducible form of NOS, iNOS (also designated NOS2), isCa2+-independent, expressed in a broad range of cell types and maps to 17cen -q12.
STORAGE
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDSrequired.
DATA
A B 201 K -
117 K 82 K -
< NOS2
NOS2 (N-20): sc-651. Western blot analysis of NOS2 expression in uninduced (A) and LPS/IFN-γ stimulated (B) RAW 264.7 whole cell lysates.
NOS2(N-20): sc-651. Immunofluorescence staining of methanol-fixed RAW 264.7 cells stimulated with LPS/IFN-γ showing cytoplasmic staining.
CHROMOSOMAL LOCATION
Genetic locus: NOS2 (human) mapping to17q11.1; Nos2 (mouse) mapping to 11 B5.
SELECT PRODUCT CITATIONS
1. Carpenter, L., et al. 2001. Protein kinase Cδ activation by interleukin-1β stabilizes inducible nitric-oxide synthase mRNA inpancreatic β-cells. J. Biol. Chem. 276: 5368-5374. 2. Buchwalow, I.B., et al. 2001. Inducible nitric oxide synthase in the myocard. Mol. Cell. Biochem. 217: 73-82. 3. Yu, J.S., et al. 2002. Induction...
NOS2 (N-20): sc-651
BACKGROUND
Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogeneticallydiverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requireshomodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have beendescribed and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including ncNOS (also designated NOS1) whose activity was firstidentified in neurons and maps at 12q24.2, and ecNOS (also designated NOS3), first identified in endothelial cells and mapping at 7q35-36. The inducible form of NOS, iNOS (also designated NOS2), isCa2+-independent, expressed in a broad range of cell types and maps to 17cen -q12.
STORAGE
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDSrequired.
DATA
A B 201 K -
117 K 82 K -
< NOS2
NOS2 (N-20): sc-651. Western blot analysis of NOS2 expression in uninduced (A) and LPS/IFN-γ stimulated (B) RAW 264.7 whole cell lysates.
NOS2(N-20): sc-651. Immunofluorescence staining of methanol-fixed RAW 264.7 cells stimulated with LPS/IFN-γ showing cytoplasmic staining.
CHROMOSOMAL LOCATION
Genetic locus: NOS2 (human) mapping to17q11.1; Nos2 (mouse) mapping to 11 B5.
SELECT PRODUCT CITATIONS
1. Carpenter, L., et al. 2001. Protein kinase Cδ activation by interleukin-1β stabilizes inducible nitric-oxide synthase mRNA inpancreatic β-cells. J. Biol. Chem. 276: 5368-5374. 2. Buchwalow, I.B., et al. 2001. Inducible nitric oxide synthase in the myocard. Mol. Cell. Biochem. 217: 73-82. 3. Yu, J.S., et al. 2002. Induction...
Leer documento completo
Regístrate para leer el documento completo.