Inte Res3

Proliferation of B cells
This unit describes procedures for measuring the capacity of purified B cells to undergo proliferation. The method centers on the use of polyclonal stimulating agents(mitogens) because these agents stimulate the majority of B cells and because the alternative (measurement of antigen-induced proliferation) requires the laborious procedures of isolating antigen-specific Bcells (which are otherwise present in too low a concentration in whole B cell populations). Cross-linking of the B cell antigen receptor, surface immunoglobulin (sIg), by specific antigen stimulatescells to proliferate prior to secreting Ig. For this purpose, monoclonal or heterologous affinity-purified anti-Ig antibodies are used. B cells can also be stimulated to proliferate byantigen-nonspecific reagents (mitogens), and it is also critical to study the role of these mitogens in B cell responses. Both of these systems have the advantage that the majority of B cells will be activated. Thefirst basic protocol describes B cell proliferation induced by two commonly used stimulants--anti-Ig antibody (either anti-IgM or anti-IgD) and lipopolysaccharide (LPS)--as measured by incorporationof [3H]thymidine into dividing cells. Alternate protocols describe other commonly used mitogens as well as other means of measuring cell proliferation.
Español
Esta unidad describe los procedimientospara la medición de la capacidad de las células B purificadas a someterse a la proliferación. Los centros de método sobre el uso de anticuerpos policlonales agentes estimulantes (mitógenos), porqueestos agentes estimulan la mayoría de las células B y porque la alternativa (medición de la proliferación inducida por antígeno) requiere los procedimientos laboriosos de aislamiento de células Bespecíficas de antígeno (que son de otro modo presente en una concentración demasiado baja en las poblaciones de células B enteros). La reticulación del receptor de antígeno de células B, inmunoglobulina...