Ion exchange chromatography principles and methods
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Publicado: 24 de marzo de 2011
Ion Exchange Chromatography
Principles and Methods
18-1114-21 Edition AA
Ion Exchange Chromatography
Principles and Methods
ISBN 91 970490-3-4
Contents
1. 2. Introduction ............................................................................................9 Ion exchange chromatography .........................................................10
The theory of ionexchange .................................................................10 The matrix.....................................................................................11 Charged groups .............................................................................13 Resolution in ion exchange chromatography .................................13 Capacity factor........................................................................15 Efficiency.................................................................................16 Selectivity.................................................................................17 Capacity ........................................................................................18
3.
Product Guide.......................................................................................20
MonoBeads ...................................................................................20 MiniBeads .....................................................................................20 SOURCE .......................................................................................20 Sepharose High Performance ion exchangers.................................21 Sepharose Fast Flow ion exchangers ..............................................21 Sepharose Big Beads ion exchangers...............................................21 STREAMLINE ion exchangers ......................................................21 DEAE Sepharose CL-6B and CM Sepharose CL-6B .......................22 DEAESephacel..............................................................................22 Sephadex ion exchangers ...............................................................22 Bulk quantities...............................................................................22 Equipment.....................................................................................22
4.
MonoBeads and MiniBeads..............................................................23 MonoBeads.....................................................................................23
Properties ......................................................................................24 Chemical stability ....................................................................24 Physical stability......................................................................25 Flow rate..................................................................................27 Capacity ..................................................................................27 Recovery..................................................................................28 Reproducibility ........................................................................29 Availability....................................................................................30 MiniBeads .......................................................................................30 Properties ......................................................................................31 Chemical stability ....................................................................31 Physical stability......................................................................32 Reproducibility ........................................................................33 Availability ....................................................................................33
2
5.
SOURCE ................................................................................................34
Properties...
Principles and Methods
18-1114-21 Edition AA
Ion Exchange Chromatography
Principles and Methods
ISBN 91 970490-3-4
Contents
1. 2. Introduction ............................................................................................9 Ion exchange chromatography .........................................................10
The theory of ionexchange .................................................................10 The matrix.....................................................................................11 Charged groups .............................................................................13 Resolution in ion exchange chromatography .................................13 Capacity factor........................................................................15 Efficiency.................................................................................16 Selectivity.................................................................................17 Capacity ........................................................................................18
3.
Product Guide.......................................................................................20
MonoBeads ...................................................................................20 MiniBeads .....................................................................................20 SOURCE .......................................................................................20 Sepharose High Performance ion exchangers.................................21 Sepharose Fast Flow ion exchangers ..............................................21 Sepharose Big Beads ion exchangers...............................................21 STREAMLINE ion exchangers ......................................................21 DEAE Sepharose CL-6B and CM Sepharose CL-6B .......................22 DEAESephacel..............................................................................22 Sephadex ion exchangers ...............................................................22 Bulk quantities...............................................................................22 Equipment.....................................................................................22
4.
MonoBeads and MiniBeads..............................................................23 MonoBeads.....................................................................................23
Properties ......................................................................................24 Chemical stability ....................................................................24 Physical stability......................................................................25 Flow rate..................................................................................27 Capacity ..................................................................................27 Recovery..................................................................................28 Reproducibility ........................................................................29 Availability....................................................................................30 MiniBeads .......................................................................................30 Properties ......................................................................................31 Chemical stability ....................................................................31 Physical stability......................................................................32 Reproducibility ........................................................................33 Availability ....................................................................................33
2
5.
SOURCE ................................................................................................34
Properties...
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