DNA EXTRACTION ACTIVITY
The objectives of this practice were playful and see the contents of what we saw on the topic: to learn and enjoy at the same time
• To extrac DNA from vegetalcells
• To observe DNA at the microscope ( despite not having given time )
What did we need?
For making this experiment, we need some material:
- One test tube
- A paperclip
- A Petri dish
- A microscope
- Filter paper
- A mortar
First, we must blend one portion of banana in a mortar and add about a cup ofwater. After that, we have to mix with salt, a spoon and 2 spoon of detergent(we only used o ne, because the spoon was so big). Gently shake the solution.The third step was filter the solution and putthe filtrated in a test tube, and carefully, pour around the same amount of alcohol into the mixture in the test tube. One of the last steps was to wait patiently for 4 minutes and observe the upperpart of the solution with some white strands or threads.
If it took too long, you could use a straightened paperclip or similar object to fish out the gooey strands of banana DNA and collect them ina Petri dish.
Finally, try to looking at DNA under a microscope
Resolution of questions:
1. What are the components of DNA molecule?
A sugar (deoxyribose), a phosphate group, and a nitrogenousbase (adenine, thymine, cytosine or guanine).
2. Where, in a cell, is located the main amount of DNA? Can we find it in another place of the cell?
Within cells, DNA is organized into long structurescalled chromosomes. During cell division these chromosomes are duplicated in the process of DNA replication, providing each cell its own complete set of chromosomes. Eukaryoticorganisms (animals, plants, fungi, and protists) store most of their DNA inside the cell nucleus and some of their DNA in organelles, such as mitochondria or chloroplasts.
3. Which structures are forming the DNA in...
Leer documento completo
Regístrate para leer el documento completo.