Los Hombres Son De Marte Y Las Mujeres De Venus
SUMMARY The aim of this study was to evaluate single and double centrifugation tube methods for concentrating equine platelets, leucocytes, transforming growth factor beta-3 (TGF-ß3) and nitric oxide (NO). Whole blood samples were collected fromclinically healthy horses and processed using single and double centrifugation tube methods to obtain four platelet concentrates (PCs): PC-A, PC-B, PC-C and PC-D, which were analyzed using a flow cytometry hematology system for haemogram. Concentrations of TGF-ß3 were determined in all the samples by ELISA and NO levels were determined by Greiss reaction. Platelet and leukocyte concentrations foreach PC and whole blood were statistically different (P < 0.05). Levels of TGF-ß3 and NO for each PC and plasma were similar. In conclusion, the single and double centrifugation tube methods are reliable for concentrating equine platelets. However, TGF-ß3 levels do not depend of the number of platelets or leucocytes concentrated with both centrifugation tube procedures. Key words: equine,platelet, transforming growth factor beta-3, nitric oxide.
Maya. S1 Rengifo. L2
ESTUDIO NIVELES DE FACTOR DE CRECIMIENTO TRANSFORMANTE BETA-3 Y ÓXIDO NÍTRICO EN CUATRO CONCENTRADOS AUTÓLOGOS DE PLAQUETAS Y PLASMA DERIVADOS DE SANGRE DE EQUINOS UBICADOS EN MEDELLIN COLOMBIA STUDY TRANSFORMING GROWTH FACTOR BETA-3 AND NITRIC OXIDE LEVELS IN FOUR AUTOLOGOUS PLATELET CONCENTRATES AND PLASMA DERIVEDFROM EQUINE BLOOD PLACE IN COLOMBIAN, COUNTRY MEDELLIN Maya. S1 Rengifo. L2 generales para producir PCs equinos: aféresis INTRODUCCION (automatizada) (Carter y col 2003), buffy Se ha propuesto la utilización de coat (semiautomatizada), buffy coat más concentrados autólogos de plaquetas (PCs) filtro (Sutter y col 2004) y centrifugado en como tratamiento de lesiones traumáticas y tubo (manual)(Argüelles y col 2006). La degenerativas del aparato locomotor de técnica de centrifugado simple y doble en caballos (Argüelles y col 2005, Carmona y col tubo (Argüelles y col 2006) ha adquirido gran 2005, Prades y col 2006). Se piensa que la popularidad en clínica equina, ya que es un gran cantidad de factores de crecimiento procedimiento fácil de realizar y es muy (GFs) liberados a dosis...
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