Pach clamping

Patch Clamping: An Introductory Guide To Patch Clamp Electrophysiology. Areles Molleman Copyright 2003 John Wiley & Sons, Ltd. ISBN: 0-471-48685-X

Patch Clamping

Patch Clamping
An Introductory Guide to Patch Clamp Electrophysiology

Areles Molleman
University of Hertfordshire, UK

Copyright # 2003

John Wiley & Sons Ltd, The Atrium, Southern Gate, Chichester, West Sussex PO19 8SQ,England Telephone (+44) 1243 779777

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Library of Congress Cataloging-in-Publication Data Molleman, Areles. Patch clamping : an introductory guide to patch clamp electrophysiology / Areles Molleman. p. cm. Includes bibliographical references and index. ISBN 0 471 48685 X 1. Patch-clamp techniques (Electrophysiology) I. Title. QP341.M6842002 572’.437 — dc21 2002027413 British Library Cataloguing in Publication Data A catalogue record for this book is available from the British Library ISBN 0 471 48685 X Paperback Typeset in 11/131 Sabon by Keytec Typesetting Ltd 2 Printed and bound in Great Britain by TJ International, Padstow, Cornwall This book is printed on acid-free paper responsibly manufactured from sustainable forestry, inwhich at least two trees are planted for each one used for paper production.

Contents

Preface 1
1.1

ix 1
1

Introduction
Patch Clamping and its Context

2
2.1

Basic Theoretical Principles
Introduction to Membrane Biology
2.1.1 2.1.2 2.1.3 2.1.4 The plasma membrane and its ionic environment Electrochemical gradients and the Nernst equation Maintenance of ion gradients and themembrane potential Ion channels Driving force and membrane resistance Membrane capacitance Consequences of membrane capacitance An electronic model of the plasma membrane The basics of equivalent circuits Intracellular recording Voltage clamp and current clamp Introduction to patch clamp configurations The equivalent circuit for the cell-attached patch configuration The equivalent circuit for thewhole-cell configuration The equivalent circuit for the excised patch configurations

5
5
5 7 8 11

2.2

Electrical Properties of the Cell Membrane
2.2.1 2.2.2 2.2.3 2.2.4

13
13 15 16 17

2.3

Recording Modes and their Equivalent Circuits
2.3.1 2.3.2 2.3.3 2.3.4 2.3.5 2.3.6 2.3.7

18
18 22 28 30 35 39 40

3
3.1

Requirements
The Platform
3.1.1 3.1.2 3.1.3 Stability:...