Papaya

Certificate of Analysis
RQ1 RNase-Free DNase (Cat.# M6101):
Contents
Part No.
M610A
M199A
M198A

Name
RQ1 RNase-Free DNase
Stop Solution
RQ1 DNase 10X Reaction Buffer

Size (units)1,000
1ml
1ml

Storage Conditions: Store at –20°C. Avoid exposure to frequent temperature changes. See the expiration date on the
Product Information Label.
Description: RQ1 (RNA-Qualified)RNase-Free DNase is a DNase I (endonuclease) that degrades both double-stranded and
single-stranded DNA, producing 3´-OH oligonucleotides (1). (RQ1 RNase-Free DNase may be used in applications wheremaintaining RNA integrity is critical.) This DNase is suited for applications such as nick translation (2), production of random fragments (3), cleavage of genomic DNA for footprinting (3), removal of DNAtemplate after in vitro transcription (4) and
removal of DNA from RNA samples prior to applications such as RT-PCR (5).

Part# 9PIM610
Revised 1/09

A F 9 P I M6 1 0

0 1 0 9 M6 1 0

UnitDefinition: One unit of RQ1 RNase-Free DNase is defined as the amount required to completely degrade 1µg of lambda
DNA in 10 minutes at 37°C in 50µl of a buffer containing 40mM Tris-HCl (pH 7.9), 10mMNaCl, 6mM MgCl2 and 10mM CaCl2.
See the unit concentration on the Product Information Label.
Biological Source: Bovine pancreas.
Requirement: Ca2+ and Mg2+ or Mn2+ (6).
Inhibitors: EGTA; EDTA (6);salt concentrations >100mM will reduce DNase activity.
RQ1 DNase 10X Reaction Buffer (M198A): 400mM Tris-HCl (pH 8.0), 100mM MgSO4 and 10mM CaCl2.
Stop Solution (M199A): 20mM EGTA (pH 8.0).
EnzymeStorage Buffer: RQ1 DNase is supplied in 10mM HEPES (pH 7.5), 50% glycerol (v/v), 10mM CaCl2 and
10mM MgCl2.
Usage Notes:
1. This DNase solution does not contain an RNase inhibitor. Observe cautionin handling the product to avoid contaminating
it with RNase.

Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711-5399
USA
Telephone
608-274-4330
Toll Free
800-356-9526
Fax...