Paper1

OriginalArticle
Cytogenet Genome Ptes12l:27I-276 (2008) DOI: 10.1159/000138896

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sole of satellite thewedge centromer¡c The (D col gogI osso neota,Pleuronectiformes) cu i o mot¡f of mainly a sequence iscomposed DNAs Gentromeric in conserved othervertebrate
M' R.de la HerránuF.RoblesuJ.l.Navasbl. López-Floresu Herrerab Rejónu Rejónu M. Ruiz C. Garrido-Ramosu Ruiz l.HacherobM.A.
"Departamento de Genética, Facultad de ciencias, universidad de Granada, Granada, (spain) tlrÍpeé..1t. Á*"" ¿a pino, consejería de Innovación, ciencia y EmPresa'cartaya Accepted in revised form for publication by M' Schmid, 9 April 2008'

Abstract. Here, a new satellite-DNA family is isolated cuneata Dicologoglossa from wedgesole, and characterized a fish havinga smallge(Pleuronectiformes),Moreau,1881 was nome.This satellite-DNAfamily of sequences isolated after digestion of,genomic DNA by conventionalcloning unitsare 171bpin Repeat enzyme. iithth" DroI restriction a high AT content (63%).Severalruns of conlength with ,..itiu" adeninei and thymines werefound, and concomitantly computer analysesrevealedthat these regions are curvature' Espeprottbto acquirestablesequence-directed areiiully r"-utkable is that tñe DraI sequences composed almóstentirely of the repetition of up to fourteen9-bp mo-

visiae reveala possiblerole in centromerefunction' All provide important information on the thesecharacteristics origin, function, andlhe evolutionof the centromericsatelCopyright @2008 S. Karger AG, Basel lite DNAs in wedgesole.

(Charlesand regionssuchasthoseof centromerestelomeres worth etal.,1994). family in euof The presence at leastone satellite-DNA implies possibleimportance of such karyotii centromeres ,"qú"n... in cell division. However' it is still debatable whether centromeric satelliteDNA has a definitive functional role (Choo,2000;Henikoff et al., 2001;Plohl et al'' conservation, of 2008).Primarily due to absence sequence givethecentromericsatellite-DNAsequencmany features function' Thus, in tó esthe chance participate centromere to date,thereis refor all centromericsateliiteDNAs found ofstructural featuressuchasrepeat markableconservation
Thi'**kl'"'b*"f-,ndedbyprojectINIARTA2005.002|5-c03cofinanced with FEDERfunds. Requestreprints from Roberto de la Herrán -Depariamento de Genética, Facultad de Ciencias Universidad de Granada Granada(Spain)s/n.,ES-18071 Avda. Fuentenueva telephone:+34 958243080;fax: +34 959244073 e-mail: rherran@ugr.es

motive' box, a17-bp of bilities.That is the case the CENP-B

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The CENP-Bsequence motifswerefirst described mamin malian centromeric satellite DNA (humanandmice)asbeing a binding acceptorfor the CENP-Bprotein and playing a role in controlling centromereformation (Earnsháwand Rothfield,1985;Masumotoet al., 1989;Haaf et al., L992; Muro et al., 1992;Kipling et a1.,1995;Ohzeki et al., 2002; Okadaet aL'2007).Álso, there are other centromeric se, quencemotifs involved in the binding of centromericpro- enzyme. ECLTM direct nucleic acid labeling method anddetection systemwere used to reveal hybridization signals following the manuteins,suchasan alternative the CENp-Bbox in human, facturer'sinstructions (Amersham to Bioscienies). motif pJcr, which is recognized alphasatellite-binding by proteinplcr (Gaffet a1.,1994; Romanova al., 1996) seet or quences motifs, suchas CDEIII, conserved the centroin meres of Saccharomyces (Clarke and Carbon, cerevisiae1985). this sense, In several satellite DNAs showingCENPB box and CDEIII sequence motifs havebeenfouná in other species yertebrates of (Garrido-Ramos al., 1995; et RagFor the sequence analysis,multiple alignments wereperformed usghianti et al., 1995; Capriglione al., 1998), et invertebratés in_g ClustalX (Thompson et al., 1997)followed by manual adjustments(López Edstrom, and 1998; Canapa...