Quimico

BD Phoenix™ ID/AST
Manual Panel Inoculation
1

2

Open panel package.
InOculATe wIThIn
2 hOurS.

4

Prepare Purity Plate.
Using a sterile loop,
recover a small drop from
theinoculum fluid either
before or after inoculating
the panel. Inoculate an agar
plate (any appropriate medium)
for purity check. Incubate plates
for 18 - 24 hours or 18 - 48 hours
foryeast, at 35°C under appropriate
conditions.

Prepare Phoenix™ ID Broth.
Inoculate panel with prepared
_
ID Broth within (< ) 60 mInuTeS.
PrePAre ID BrOTh.

After panel inoculation,place closures
securely on the panel to seal.

5

load panel(s) into BD Phoenix instrument
_
within (< ) 30 mInuTeS after inoculation.

• Add organism to ID Broth.
• Vortex.
•Make .50 - .60 McFarland
(Standard inoculum)*.
OR
• Make .20 - .30 McFarland
(Low inoculum)**.
OR
• Make 2.00 - 2.40 McFarland
(Yeast inoculum).

3

Prepare Phoenix™ AST or AST-SBroth.
(ID/AST, AST Panels only)
_
Inoculate panel with prepared broth within (< ) 30 mInuTeS.
PrePAre AST or AST-S BrOTh***
• Add one free falling drop of AST or AST-S Indicator****
intoAST or AST-S Broth. Tube can be used for up to
2 hours if exposed to light or up to 8 hours in the dark.
Mix by inverting.
• Add 25 mL prepared ID Broth
(Standard inoculum).
OR
• Add50 mL prepared ID Broth
(Low inoculum).

* Not applicable for yeast ID panels.
** Not applicable for strep or yeast ID panels.
*** For gram-negative or gram-positive panels
use PhoenixAST Indicator and AST Broth.
For strep panels use Phoenix AST-S Indicator and AST-S Broth.
**** AST or AST-S indicator is good for 14 days after opening (refrigerated).

BD Diagnostics7 Loveton Circle
Sparks, MD 21152-0999
800.638.8663
www.bd.com/ds
BD, BD Logo and Phoenix are trademarks of
Becton, Dickinson and Company. ©2012 BD
2-222341 January 2012
SHQ-226