Rataas

Animal Feed Science and Technology 149 (2009) 265–274

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Animal Feed Science and Technology
journal homepage: www.elsevier.com/locate/anifeedsciAssociation of Lactobacillus buchneri with aerobic stability of total mixed ration containing wet brewers grains preserved as a silage
Fujin Wang, Naoki Nishino ∗
Department of Biomolecular Science,Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan

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Laboratory-scale plastic silos were prepared to examinebacterial factors associated with the aerobic stability of total mixed rations (TMR) preserved as a silage after mixing. In experiment 1, TMR silages containing wet brewers grains (BG), hay (lucerne orsudangrass), maize grain, wheat bran, beet pulp and liquid molasses, were ensiled for 14 and 56 days, and bacterial communities were examined by denaturing gradient gel electrophoresis (DGGE). Thesilages resisted aerobic deterioration for as long as 2 weeks, except that heating occurred after 5.5 days in 14-day silage prepared with sudangrass hay. The DGGE analyses indicated that, although not foundin the materials, Lactobacillus buchneri and L. brevis were detectable in long-stored TMR silage. L. delbrueckii and L. fermentum were found in the materials, and appeared to survive duringfermentation and after exposure to air. There were few changes in the DGGE profiles before and after heating in deteriorated silage containing sudangrass hay. In experiment 2, TMR silage with ingredientssimilar to those in experiment 1 was prepared, and culturable lactic acid bacteria were isolated from the plates of de Man-RogosaSharpe agar. L. delbrueckii and L. fermentum were obtained from the materialsbut not from TMR silage. L. buchneri and L. brevis were not found in the materials, but more than one-third of the isolates were L. buchneri and L. brevis in 14- and 56-day silages, respectively....