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González Guerrero, D.; Esparza Martínez, V.; Torre Almaráz, R. de la CULTIVATION OF TRAMETES VERSICOLOR IN MEXICO Micología Aplicada Internacional, vol. 23, núm. 2, 2011, pp. 55-58 Colegio de Postgraduados Puebla, México
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Micología Aplicada Internacional ISSN (Printed Version): 1534-2581 dcarrera@colpos.mx Colegio de Postgraduados México

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micologia Cultivation of TrameTes23(2), 2011, pp. 55-58aplicada inTernaTional, versicolor 55
© 2011, Berkeley, CA, U.S.A. www.micaplint.com

Cultivation of TrameTes versicolor in MexiCo
D. González Guerrero, v. esparza Martínez anD r. De la torre alMaráz
Universidad Nacional Autónoma de México (UNAM), Facultad de Estudios Superiores Iztacala (FESI), Avenida De los Barrios, Colonia Reyes Iztacala, Tlalnepantla 54090, Estado de México, Mexico. Correoelectrónico: vmem69@gmail.com Accepted for publication June 29, 2011

ABSTRACT A native strain of Trametes versicolor (Coriolaceae) was isolated and cultivated under laboratory conditions. Mycelial colonies were off-white, showing high density, velvety texture, and abundant aerial hyphae. Substrates studied had good mycelial growth and colonization. Higher mushroom yield of 173.8 g was recorded onsupplemented oak sawdust, reaching a biological efficiency of 20.3%. Leathery, dark brown fruit bodies were obtained having normal morphology. A lower biological efficiency of 3.2% was reached on not supplemented oak sawdust. Key words: Cultivation, Trametes versicolor, oak substrates.

INTRODUCTION Trametes versicolor (L.) Lloyd has been considered among the 25 major medicinal macrofungiworldwide1, mainly due to its traditional usage. Interesting polysaccharopeptides have been purified from this species, showing experimental immunomodulatory and anti-cancer effects3,4. Strains from T. versicolor have

also been used in bioremediation studies6. In this study, we isolated a wild strain of T. versicolor, which was cultivated on oak sawdust substrate, either not supplemented orsupplemented. MATERIALS AND METHODS A strain of Trametes versicolor was

micol. apl. inT., 23(2), 2011, pp. 55-58

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D. González Guerrero eT al.
Culture media. Malt extract agar (MEA, Bioxon) and potato dextrose agar (PDA, Bioxon) were used for strain characterization. Commercial culture media were prepared and sterilized according to manufacturer instructions. Sterile culture media (20 ml/petridish of 90 mm diameter) were inoculated with the strain of T. versicolor, and incubated at 24±1 C. Five replicates were made per culture medium. Mycelial growth rate and colony morphology were assessed by simple observations recorded every two or three days. Mushroom cultivation. Sorghum grain spawn was prepared in glass jars according to standard methods2. Sterile jars containing 250 g ofsorghum kernels were inoculated with T. versicolor, and incubated at 24±1 C until complete mycelial colonization. The following substrates were studied (dry weight): oak sawdust, and supplemented oak sawdust (homogeneous mixture: 78% oak sawdust, 10% sorghum kernels, 10% wheat bran, 2% gypsum). About a kilogram (856 g dry weight) of each substrate was placed within polypropylene plastic bags (15 x 39cm), adjusting moisture content to 60-70%. These bags were then sterilized at 121 C for 2 h. Each bag was homogeneously inoculated with 70-80 g of spawn under sterile conditions, and incubated at 22 C in the dark. Five replicate bags were done for each substrate. When the substrates were colonized by the mushroom mycelium, environmental factors were adjusted to promote fruiting (ventilation,...