Replicacion de dna

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REPLICACIÓN DEL DNA.
–Modelos: La replicación es semiconservativa(ni conservativa ni dispersa). –El replicón. –El replicón: la unidad replicativa. –Cadenas molde(template), cebadores (primers), primosomas y replisomas: primasas, ligasas, girasas, helicasas, topoisomerasas y polimerasas. –La síntesis es discontinua, Fragmentos precursores o de Okasaki y ocurre en dirección 5`- 3`.

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REPLICACIÓN DEL DNA.
–DNA DNA Dos cadenas antiparalelas y complementarias El modelo de Watson y Crick sugiere: Replicación conservativa. Replicación semiconservativa Replicación Dispersa

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MODELO SEMICONSERVATIVO.

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E. Coli. En división Duplicación: INICIACIÒN. ELONGACIÓN TERMINACIÓN.

Bacterias

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REPLICACIÓN DEL DNA.
Cromosoma Bacteriano.Remocion de Nucleosomas en eucariontes.

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ORIGEN DEREPLICACIÓN.

Origen de replicación en E coli Ori C 245pb ricas en A-T. Replicación bidireccional. Estructura θ (theta).

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CADENA LIDER ( REPLICACIÓN CONTINUA) CADENARETARDADA (REPLICACIÓN DISCONTINUA FRAGMENTOS DE OKASAKI)

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Replicón.
REPLICÓN.- DNA replicado, el origen de replicación y las proteínas involucradas: •Polimerasas,•Primer, •Helicasas (horquilla de replicación), •Toposiomerasas, •Helicasas

Componentes requeridos para duplicación.

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CADENAS MOLDE.

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DNA POLIMERASAS.
•Descubiertas por A. Kornerberg usando extractos de E. coli 3´
5´ 5´ 3´

Extiende la cadena a partir de un grupo hidroxilo 3´, mediante ataque nucleofilico, utiliza 2 fosfatoa por...