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Publicado: 3 de marzo de 2013
ISOLATION OF INORGANIC PHOSPHATE SOLUBILIZING BACTERIA IN THE RHIZOSP HERE OF
TOMATO CROPS IN YUCATAN
Enoc Cetina-Mancilla, Geovani Garrido-Itza, Eduardo Burgos-Valencia, Diana Escalante -Réndiz;Universidad Autónoma de
Yucatán; Facultad de Ingeniería Química; Campus de Ingenierías y Ciencias Exactas; Mérida, Yucatán, México;
l_pax@hotmail.com
Key words: Bacillus sp., inorganic phosphate,microbial growth
Introduction. Phosphorus plays an important role in all
processes required in plant nutrition. The availability of this
element in the soil is low, because the phosphorus is in
theform of insoluble salts. By using biofertilizers, the
phosphorus can be exploited by plants. Species of the
genus Bacillus have this property, so they can be used as
plant growth promotingbacteria.
The aim of this work was to obtain inorganic phosphate
solubilizing bacteria belonging to the genus Bacillus from a
tomato crop in the state of Yucatan, for possible use as
biofertilizer.
)Fig. 2. a) Gram stain test. APP isolate was Gram-positive
bacilli bacteria. b) APP isolate with presence of endospores.
Spores are observed green.
APP 10%
3.6
L
o
g
F
C
U
)
m
i
ll
o
n
s
/
m
L
3.4
3.2
3
2.8
2.6
2.4
2.2
)
Results. Six inorganic phosphate solubilizing isolates
were obtained (Fig. 1). One of them, called APP was
found to be Gram -positivebacillus and endospores
presence (Fig. 2). The biochemical test results were in
accordance to the Bacillus genus (2). The culture of APP
with 10% inoculum concentration had a growth rate of
90.1952 generations / hr, reaching a population of 10 CFU
/ mL at 10 hours of bacterial growth. The amount is
suitable for testing biofertilization in plants (3).
b
)
(
Methodology. Soilsamples were taken from a tomato
crop in a greenhouse of Too Yucatan region. Decimal
dilutions were performed on samples, which were
subjected to 80 ° C for 15 min. Subsequently the samples
were...
TOMATO CROPS IN YUCATAN
Enoc Cetina-Mancilla, Geovani Garrido-Itza, Eduardo Burgos-Valencia, Diana Escalante -Réndiz;Universidad Autónoma de
Yucatán; Facultad de Ingeniería Química; Campus de Ingenierías y Ciencias Exactas; Mérida, Yucatán, México;
l_pax@hotmail.com
Key words: Bacillus sp., inorganic phosphate,microbial growth
Introduction. Phosphorus plays an important role in all
processes required in plant nutrition. The availability of this
element in the soil is low, because the phosphorus is in
theform of insoluble salts. By using biofertilizers, the
phosphorus can be exploited by plants. Species of the
genus Bacillus have this property, so they can be used as
plant growth promotingbacteria.
The aim of this work was to obtain inorganic phosphate
solubilizing bacteria belonging to the genus Bacillus from a
tomato crop in the state of Yucatan, for possible use as
biofertilizer.
)Fig. 2. a) Gram stain test. APP isolate was Gram-positive
bacilli bacteria. b) APP isolate with presence of endospores.
Spores are observed green.
APP 10%
3.6
L
o
g
F
C
U
)
m
i
ll
o
n
s
/
m
L
3.4
3.2
3
2.8
2.6
2.4
2.2
)
Results. Six inorganic phosphate solubilizing isolates
were obtained (Fig. 1). One of them, called APP was
found to be Gram -positivebacillus and endospores
presence (Fig. 2). The biochemical test results were in
accordance to the Bacillus genus (2). The culture of APP
with 10% inoculum concentration had a growth rate of
90.1952 generations / hr, reaching a population of 10 CFU
/ mL at 10 hours of bacterial growth. The amount is
suitable for testing biofertilization in plants (3).
b
)
(
Methodology. Soilsamples were taken from a tomato
crop in a greenhouse of Too Yucatan region. Decimal
dilutions were performed on samples, which were
subjected to 80 ° C for 15 min. Subsequently the samples
were...
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