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The sodium-activated sodium channel is expressed in the
rat kidney thick ascending limb and collecting duct cells
and is upregulated during high salt intake

Lucienne S. Lara, Ryousuke Satou, Camille R. T. Bourgeois, Alexis A. Gonzalez, Andrea
Zsombok, Minolfa C. Prieto and L. Gabriel Navar
Am J Physiol Renal Physiol 303:F105-F109, 2012. First published 21 March 2012;
doi:10.1152/ajprenal.00490.2011
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Am J Physiol Renal Physiol 303:F105–F109, 2012.
First published March 21, 2012; doi:10.1152/ajprenal.00490.2011.

The sodium-activated sodium channel is expressed in the rat kidney thick
ascending limb and collecting duct cells and is upregulated during high
salt intake
Lucienne S. Lara,1,2,3 Ryousuke Satou,1,2 Camille R. T. Bourgeois,1 Alexis A. Gonzalez,1,2
Andrea Zsombok,1 Minolfa C. Prieto,1,2 and L. GabrielNavar1,2
1

Department of Physiology, Tulane University School of Medicine and 2Hypertension and Renal Center of Excellence, Tulane
University School of Medicine, New Orleans, Louisiana; and 3Instituto de Ciencias Biomedicas, Universidade Federal do Rio
de Janeiro, Rio de Janeiro, Brazil

Lara LS, Satou R, Bourgeois CR, Gonzalez AA, Zsombok A,
Prieto MC, Navar LG. The sodium-activated sodiumchannel is
expressed in the rat kidney thick ascending limb and collecting duct
cells and is upregulated during high salt intake. Am J Physiol Renal
Physiol 303: F105–F109, 2012. First published March 21, 2012;
doi:10.1152/ajprenal.00490.2011.—Increased dietary salt triggers oxidative stress and kidney injury in salt-sensitive hypertension; however, the mechanism for sensing increasedextracellular Na concentration ([Na ]) remains unclear. A Na -activated Na channel (Na
sensor) described in the brain operates as a sensor of extracellular
fluid [Na ]; nonetheless, its presence in the kidney has not been
established. In the present study, we demonstrated the gene expression
of the Na sensor by RT-PCR and Western blotting in the SpragueDawley rat kidney. Using immunofluorescence, the Nasensor was
localized to the luminal side in tubular epithelial cells of collecting
ducts colocalizing with aquaporin-2, a marker of principal cells, and
in thick ascending limb, colocalizing with the glycoprotein TammHorsfall. To determine the effect of a high-salt diet (HSD) on Na
sensor gene expression, we quantified its transcript and protein levels
primarily in renal medullas from controlrats and rats subjected to 8%
NaCl for 7 days (n 5). HSD increased Na sensor expression levels
(mRNA: from 1.2 0.2 to 5.1 1.3 au; protein: from 0.98 0.15
to 1.74
0.28 au P
0.05) in the kidney medulla, but not in the
cortex. These data indicate that rat kidney epithelial cells of the thick
ascending limb and principal cells of the collecting duct possess a Na
sensor that is upregulated by...