Pge2

Parameter™
PGE2 Assay
Catalog Number KGE004

For the quantitative determination of Prostaglandin E2 (PGE2) in cell culture supernates, serum, plasma, and urine.

This package insert must be read in its entirety before using this product.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

TABLE OF CONTENTS
Contents Page
INTRODUCTION 2 PRINCIPLE OF THE ASSAY . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 LIMITATIONS OF THE PROCEDURE 3 TECHNICAL HINTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 REAGENTS 4 STORAGE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 OTHER SUPPLIES REQUIRED 5 PRECAUTIONS . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . 5 SAMPLE COLLECTION AND STORAGE 5 SAMPLE PREPARATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 SAMPLE EXTRACTION 6 REAGENT PREPARATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 ASSAY PROCEDURE, REGULAR SENSITIVITY ASSAY OPTION 8 ASSAY PROCEDURE, HIGH SENSITIVITYASSAY OPTION . . . . . . . . . . . . . . . . . . . . . . 9 ASSAY PROCEDURE SUMMARY, REGULAR SENSITIVITY ASSAY OPTION 10 ASSAY PROCEDURE SUMMARY, HIGH SENSITIVITY ASSAY OPTION . . . . . . . . . . . . . . . 11 CALCULATION OF RESULTS 12 TYPICAL DATA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 PRECISION 13 RECOVERY . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . 13 LINEARITY 14 SENSITIVITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 SAMPLE VALUES 15 ASSAY CORRELATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 SPECIFICITY 16 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . 17 PLATE LAYOUT 18

MANUFACTURED AND DISTRIBUTED BY:
R&D Systems, Inc. 614 McKinley Place NE Minneapolis, MN 55413 United States of America TELEPHONE: FAX: E-MAIL: (800) 343-7475 (612) 379-2956 (612) 656-4400 info@RnDSystems.com

DISTRIBUTED BY:
R&D Systems Europe, Ltd. 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB United Kingdom R&D Systems GmbH Borsigstrasse 765205 Wiesbaden-Nordenstadt Germany R&D Systems Europe 77 boulevard Vauban 59041 LILLE CEDEX France TELEPHONE: FAX: E-MAIL: +44 (0)1235 529449 +44 (0)1235 533420 info@RnDSystems.co.uk

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+49 (0)6122 90980 +49 (0)6122 909819 infogmbh@RnDSystems.co.uk +0800 90 72 49 +0800 77 16 68 info@RnDSystems.co.uk

INTRODUCTION
Prostaglandins (PG),thromboxanes, and leukotrienes belong to the class of prostanoid fatty acid derivatives of arachidonic acid. Arachidonic acid is liberated from membrane phospholipids by the action of phospholipases, metabolized into PGG 2 and PGH2 by the cyclooxygenases COX-1 and COX-2, and converted into PGE 2 by prostaglandin E synthetase (PGES) (1 - 3). PGE2 is found in many bodily fluids and is inactivated in the lungsand liver by prostaglandin dehydrogenase and cytochrome P-450 monooxygenases. COX and PGES exist in different isoforms that are constitutively active or inducible by inflammatory stimuli. PGE2 synthesis can be blocked by corticosteroids that inhibit the phospholipases or by nonsteroidal anti-inflammatory drugs (NSAIDs) that inhibit the cyclooxygenases (4, 5). PGE 2 is produced by a wide variety oftissues, including bronchiolar, gastrointestinal, vascular, uterine, and bladder smooth muscle, fetal ductus arteriosus, placenta, brain, renal macula densa cells, testicular Leydig cells, mesenchymal stem cells, monocytes, and macrophages (6 - 17). Increased amounts of PGE2 are produced in several pathologic conditions, including inflammation and arthritis, fever, tissue injury, endometriosis,...