1Cultivo_Cel_Mater_y_Esteriliza_Medios

CULTIVO DE
CÉLULAS ANIMALES

TABLE 1.1. Key Events in the Development of Cell and Tissue Culture Date Event Reference
1907 Frog embryo nerve fiber outgrowth in vitro.

Harrison, 1907

1912 Explants of chick connective tissue; heart muscle contractile for 2–3 months

Carrel, 1912; Burrows, 1912

1916 Trypsinization and subculture of explants

Rous & Jones, 1916

1920s/30sSubculture of fibroblastic cell lines

Carrel & Ebeling, 1923

1925–1926 Differentiation in vitro in organ culture

Strangeways & Fell, 1925, 1926

1940s Introduction of use of antibiotics in tissue culture

Keilova, 1948; Cruikshank & Lowbury, 1952

1943 Establishment of the L-cell mouse fibroblast; first continuous cell line

Earle et al., 1943

1948 Cloning of the L-cell

Sanford etal., 1948

1949 Growth of virus in cell culture

Enders et al., 1949

1952 Use of trypsin for generation of replicate subcultures

Dulbecco, 1952

Virus plaque assay

Dulbecco, 1952

1952–1955 Establishment the first human cell line, HeLa, from a cervical carcinoma,

Gey et al., 1952

1952 Nuclear transplantation see

Briggs & King, 1960

1954 Fibroblast contact inhibition ofcell motility

Abercrombie & Heaysman, 1954
Griffiths, 1991

Salk polio vaccine grown in monkey kidney cells see
1955 Cloning of HeLa on a homologous feeder layer

Puck & Marcus, 1955
Eagle, 1955, 1959

Development of defined media
Requirement of defined media for serum growth factors
Realization of importance of mycoplasma (PPLO) infection

Sanford et al., 1955; Harris, 1959, Late1950s
Coriell et al., 1958; Rothblat & Morton,1959;Nelson, 1960

1961 Definition of finite life span of normal human cells
Cell fusion–somatic cell hybridization
1962 Establishment and transformation of BHK21
Maintenance of differentiation (pituitary & adrenal tumors)

Hayflick & Moorhead, 1961
Sorieul & Ephrussi, 1961
Macpherson & Stoker, 1962
Buonassisi et al., 1962; Yasamura et al.,1966;
Sato & Yasumura, 1966

1963 3T3 cells & spontaneous transformation

Todaro & Green, 1963

1964 Pluripotency of embryonal stem cells

Kleinsmith & Pierce, 1964

Selection of transformed cells in agar

Macpherson & Montagnier, 1964

1964–1969 Rabies, Rubella vaccines in WI-38 human lung fibroblasts ____Wiktor et al., 1964; Andzaparidze, 1968
1965 Serum-free cloning of Chinesehamster cells

Ham, 1965

Heterokaryons—man–mouse hybrids

Harris & Watkins, 1965

1966 Nerve growth factor

Levi-Montalcini, 1966

Differentiation in rat hepatomas

Thompson et al., 1966

1967 Epidermal growth factor

Hoober & Cohen, 1967

HeLa cell cross-contamination

Gartler, 1967

Density limitation of cell proliferation

Stoker & Rubin, 1967

Lymphoblastoid celllines Moore et al., 1967;

Gerper et al., 1969; Miller et al., 1971

1968 Retention of differentiation in cultured normal myoblasts

Yaffe, 1968

Anchorage-independent cell proliferation

Stoker et al., 1968

1969 Colony formation in hematopoietic cells
1970s Development of laminar-flow cabinets see

Metcalf, 1969; see also Metcalf, 1990
Kruse et al., 1991; Collins & Kennedy,19991973 DNA transfer, calcium phosphate

Graham & Van der Eb, 1973

1975 Fibroblast growth factor

Gospodarowicz et al., 1975

Hybridomas—monoclonal antibodies

Kohler & Milstein, 1975

1976 Totipotency of embryonal stem cells

Illmensee & Mintz, 1976

Growth factor-supplemented serum-free media

Hayashi & Sato, 1976

1977 Confirmation of HeLa cell cross-contamination of manycell lines
3T3 feeder layer and skin culture
1978 MCDB-selective, serum-free media
Matrix interactions
Cell shape and growth control

Nelson-Rees & Flandermeyer, 1977
Rheinwald & Green, 1975
Ham & McKeehan, 1978

Gospodarowicz et al., 1978b; Reid & Rojkind, 1979
Folkman & Moscona, 1978

Date

Event

1980s

Regulation of gene expression
Oncogenes, malignancy, and transformation...