Candida

Brazilian Journal of Microbiology (2011) 42: 1093-1100
ISSN 1517-8382

MEDIUM COMPOSITION INFLUENCE ON BIOTIN AND RIBOFLAVIN PRODUCTION BY NEWLY ISOLATED
CANDIDA SP.
Gaby Tiemi Suzuki, Juliana Alves Macedo*, Gabriela Alves Macedo
Departamento de Ciência de Alimentos, Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas, Campinas,
SP, Brasil.
Submitted: October 16, 2009;Returned to authors for corrections: December 10, 2010; Approved: May 16, 2011.

ABSTRACT
Complex B vitamins as Biotin and Riboflavin are required by living organisms, not only for growth but also
for metabolite production, and the feed market classifies them as growth promoters. Since Brazil will soon
be one of the world’s biggest animal protein producers, feed production is a largeconsumer of vitamins and
micronutrients. The industry requires 10 mg riboflavin/0.2 mg biotin per kilogram of feed; a ratio of 40 ~
50:1. Although few studies have been conducted specifically on riboflavin production using factorial design
and surface response method as an optimization strategy, it is a common practice in biotechnology with
many research reports available. However, there are noreports on the use of statistical design for biotin
production. This study set out to evaluate medium composition influence on biotin and riboflavin production
using a statistical design. There are no studies relating biotin and riboflavin production by Candida sp LEB
130. In this preliminary study to improve the simultaneous production of biotin and riboflavin, the maximum
riboflavin/biotin ratioof 8.3 µg/mL was achieved with medium component concentrations of: sucrose 30
g/L, KH2PO4 2 g/L, MgSO4 1 g/L and ZnSO4 0.5mL/L.
Key words: Candida sp., riboflavin, biotin, media development, vitamin production
INTRODUCTION

production. Ashbya gossypii, for instance, needs many nutrients
for riboflavin production, biotin among them (2). In a

Although work reported during recent years hasled to a

preliminary study, most of the isolate strains produced biotin

nearly complete understanding of the metabolic role of biotin,

and riboflavin simultaneously with a maximum ratio of 15 µg

comparatively little is known about the biosynthesis of this B-

riboflavin/µg biotin. In the study of Özbas and Kutsal (19),

group vitamin largely because living cells usually synthesizemaximum riboflavin production was achieved by Ashbya

extremely small amounts of it or of compounds with biotin-like

gossypii with a D-biotin concentration of 0.4 µg/L, yielding a

activity (24).

riboflavin

concentration

of

0.6

kg/g

initial

carbon

Biotin is required by a variety of yeasts, fungi and

concentration. Kojima et al, 1972 (12) also used a specificbacteria, not only for growth but also for metabolite

medium containing glucose with salts and biotin added to study

*Corresponding Author. Mailing address: Food Science Department, Faculty of Food Engineering, UNIVERSIDADE ESTADUAL DE CAMPINAS
(UNICAMP) P.O. Box 6121, CEP 13083-862, SP, Brazil.; Tel.: +55 19 3521-2175 Fax: +55 19 35212153.; E-mail: jumacedo@gmail.com

1093

Suzuki,G.T. et al.

Biotin and riboflavin production by Candida sp.

riboflavin production by Eremothecium ashbyii. Kalingan and

Engineering, UNICAMP, from a soil sample collected from a

Krishnan (9) showed that the concentration of yeast

sugarcane plantation in São Paulo State It is maintained in

hydrolysate (which contains biotin and thiamin) that induced

cryotubes containing 10%glycerol at -800 C. To prepare

the highest vitamin production by Eremothecium ashbyii was

subcultures, yeast was inoculated onto Yeast Malt agar (2%

3

30 kg/m , and the specific production rate increased by 48%

glucose, 1% yeast extract, 2% peptone, 3% agar) and incubated

when compared to control. However, contrary effects were also

at 300 C for 24 h before fermentation tests....