ESTUDIO DE PARAMETROS DE DIVERSIDAD GENETICA EN CABRAS DEL DEPARTAMENTO DE PRESIDENTE HAYES PARAGUAY

ESTUDIO DE PARAMETROS DE DIVERSIDAD GENETICA EN CABRAS DEL DEPARTAMENTO DE PRESIDENTE HAYES PARAGUAY

STUDY OF THE GENETIC VARIABILITY OF POPULATIONS CAPRINAS RAISED IN CATTLE CONVENTIONAL SYSTEMS INSIDE THE PRESIDENT'S DEPARTMENT HAYES

Gabaglio J.M1, León D2, Romero L2,7, Ibarra P.2,6, Núñez L2,3, Castro L2,3, Ramírez L2,3, Oka Obara A.8, Martínez A.4,5, Landi V.4,5, Delgado J.V.4,5,Martínez O.R.5,6*
1Facultad de Ciencias Agrarias, Universidad Nacional de Asunción.
2Maestría de Ciencias en Biotecnología, CEMIT-DGICT, UNA.
3Facultad de Ciencias Veterinarias, Universidad Nacional de Asunción.
4Laboratorio de Genética Molecular Aplicada, Universidad de Córdoba, España.
5RED CONBIAND
6Centro Multidisciplinario de Investigaciones Tecnológicas de la Dirección General deInvestigación Científica y Tecnológica de la Universidad Nacional de Asunción. *criollos.pymail.com
7Instituto Paraguayo de Tecnología Agrícola.
8Profesor emerito de la Facultad de Ciencias Veterinarias, Universidad Nacional de Asunción.

Keywords:
Presidents Hayes
Paraguayan Chaco
Caprino
microsatellites

Palabras claves:
Presidente Hayes
Chaco Paraguayo
Caprinos
MicrosatelitesAbstract
The defined area for the study was the department of President Hayes, in Western Region of the Chaco. The aim was to study the variability and genetic diversity of goats breeded in the department of President Hayes and identify the different genotypes of goat populations in traditional farms. Subject of the study were 150 goats. 28 microsatellite markers were used in the procedure, DNA wasextracted from the roots of the hair and PCR process applied over it; to get alleles and studied DNA functions, GENESCAN software was developed. Typing and characterization of the alleles were obtained by using GENOTYPER program. Basic statistical measures such as: number of alleles, heterozygosis and PIC were determined by using the statistical program GENETIX. AFC was performed to determine geneticdifferentiation among individuals and populations. Genetic distances were estimated between goat populations by the Nei and Reynolds method, by using the program POPULATIONS. We have analyzed the genetic structure of individuals and populations by using the program STRUCTURE. Obtained results from the assignment of individuals test were plotted with complement DISTRUC. By analyzing the valuesobtained by the Nei method, we found the bigger genetic distance between Boer crossbred and Anglo Nubian, and the shortest distance was found among crossbred populations and Anglo Nubian. According to the assignment of each individual to the different clusters, we found that the group of Anglo Nubian had a good uniformity in their genetic structure. However, it is appreciated an interesting goatgenetic material in terms of variability and genetic diversity, located in the southern Chaco, which should motivate us for further studies with molecular markers. It is possible that this is a local zoo genetic resource or native from the region where the study was held, which would indicate the possibility that genetics is adequate to produce in the area.

Resumen
Escasos estudios genéticos decaprinos han sido realizados en Paraguay. Sin embargo, los mismos generan un mapa genético de individuos y poblaciones, nativas o importadas, y su distribución geográfica. Para esto, análisis de diversidad genética son la base, y este trabajo, tuvo como objetivo de conocer los índices de variabilidad dentro de cada población y entre poblaciones caprinas estudiadas, criadas en el departamento dePresidente Hayes. Fueron seleccionados 150 animales provenientes de 17 fincas, ubicadas en dicho departamento. Para el análisis genético fueron extraídas muestras de pelos del extremo de la cola, de cada animal seleccionado. Se buscaron caprinos del tipo criollos, de gran porte, cara larga, pelos largos, colores variados. Igualmente se emplearon caprinos de razas internacionalmente reconocidas, con...