Fisica Medica

Veterinaria México
Volumen Volume

33

Número Number

3

Julio-Septiembre July-September

2002

Artículo:

Lesiones histológicas en músculo esquelético, causadas por larvas de Eustrongylides sp (Nematoda: Dictophymatidae ) en ranas comestibles del Lago Cuitzeo, Michoacán, México
Derechos reservados, Copyright © 2002: Facultad de Medicina Veterinaria y Zootecnia, UNAM

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Notas de investigación
Lesiones histológicas en músculo esquelético, causadas por larvas de Eustrongylides sp (Nematoda: Dictophymatidae) en ranas comestibles del Lago Cuitzeo, Michoacán, México Histological lesions inskeletal muscle, caused by Eustrongylides sp (Nematoda: Dictiophymatoidae) larvae in edible frogs from Lake Cuitzeo, in the state of Michoacan, in Mexico
José Ramírez Lezama* David Osorio Sarabia**

Abstract
The aim of the present study was to inform about the microscopic lesions caused by Eustrongylides sp in the skeletal muscle of frogs from Lake Cuitzeo, located in Michoacan, Mexico, giventhat it is considered to be a potentially zoonotic parasite. 146 frogs, from four species were studied: 80 from Rana megapoda, 43 from Rana dunni, 16 from Rana catesbiana and seven from Rana verlandieri. All were euthanised with an intraperitoneal pentobarbital overdose. The total body weight, weight of the legs, gender and total length were registered. An external and internal examination wasthen carried out, using a stereoscopic microscope, and the Eustrongylides sp nematodes were removed using fine brushes. These were fixed first in 70% ethyl alcohol and then in Amman lactophenol to facilitate their observation and taxonomic identification under the microscope. Necropsies were performed on the 146 total specimens from the four species, finding 10 parasitic larval forms in the legmuscles of the Rana megapoda specimens. Therefore, skeletal muscle fragments measuring 0.1 × 0.2 cm were extracted and fixed in 10% buffered formalin with a pH of 7.2. These were processed with the routine hematoxylin-eosin technique for their inspection under the light microscope. Multiples focal hemorrhages, a moderate quantity of heterophils between muscle fibers and a few round nematodes with athin cuticle, muscle and pharynx, were observed. It is important to note that more studies must be carried out on these parasites, given that the frogs are of commercial importance for human consumption in the area, and can cause zoonosis and economic loss. Key words: HISTOLOGIC LESIONS, FROG SKELETAL MUSCLE, E USTRONGYLIDES SP LARVAE, LAKE CUITZEO, MICHOACAN, MEXICO.

Resumen
El presente estudiotiene como objetivo informar sobre las lesiones microscópicas provocadas por larvas de Eustrongylides sp en músculo esquelético de ranas en el Lago Cuitzeo, Michoacán, México, considerado un parásito
Recibido el 11 de septiembre de 2001 y aceptado el 26 de noviembre de 2001. * Departamento de Patología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, 04510,México, D. F. ** Laboratorio de Helmintología, Instituto de Biología, Universidad Nacional Autónoma de México, 04510, México, D. F.

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potencialmente zoonótico. Se revisaron 146 ejemplares de las cuatro especies: 80 de Rana megapoda, 43 de Rana dunni, 16 de Rana catesbiana y siete de Rana verlandieri, a los cuales se le aplicó la eutanasia con sobredosis de pentobarbital porvía intraperitoneal. Se registró el peso corporal, los pesos de las ancas, sexo y longitud total. Posteriormente se realizó el examen externo e interno bajo el microscopio esterescópico y fueron retirados con pinceles nematodos de Eustrongylides sp, se fijaron en alcohol etílico al 70% y posteriormente con lactofenol de Amman para su observación bajo el microscopio y determinación taxonómica; se...