Life
Páginas: 6 (1267 palabras)
Publicado: 17 de mayo de 2012
A few examples of my Research and Recent Research
La formación de proto-proteinas en los primeros estadios de la química prebiótica es un misterio sin resolver ¿Como pudo generarse el enlace peptídico entre los primeros aminoacidos en un ambiente geológico plausible?
Peptide bonds formation was attempted by thermal activation of dry amino acids from aqueous solution thatsimulated prebiotic evaporative environments. The evaporation trend of amino acids solutions shows a bifurcation and can lead to either a crystalline phase (near equilibrium) or a metastable-non crystalline phase (far from equilibrium). Only amino acids in this metastable phase are able to form peptide bonds by thermal activation at temperatures that are generated by solar radiation today. We suggestthat this metastable phase is the ideal initial material to trigger amino acid assemblage with protein-like structure because provide the driving force (supersaturation) for an intense interaction between monomers of different amino acids and allows activation of these monomers in plausible prebiotic conditions
Origins of Life and Evolution of the Biosphere, 30: 549-556. Viedma (2000)En este trabajo sintetizamos proto-proteinas en ambientes moderados y establecemos que la diferencia entre auto-organización cristalina y auto-organización orgánica (estado metastable) permite la formación del enlace peptídico.
Optical microphotographs (x12) of a set of drops from the same solution of aminoacids B after evaporation and ageing for a few days during the ageing process,we selected some crystallized drops, some metastable drops and another one partially metastable-crystalline drop. All them were heated at 85ºC for 80 days. Only aminoacids in metastable state formed peptide bonds.
CÓMO CRECER BUENOS CRISTALES DE PROTEÍNAS Y POR QUÉ
Para conocer la estructura tridimensional de una proteína y por tanto para conocer con exactitud sufunción biológica hay que obtener cristales para estudiarlos mediante rayos X. Obtener cristales de proteínas es muy difícil por la complejidad fisicoquímica de las interacciones a escala molecular. En este trabajo desarrollamos una técnica de cristalización de proteínas que soslaya todos los problemas planteados y ha generado los mejores cristales obtenidos hasta ahora en la TierraThis paper provides a detailed description of a simple method to obtain large protein single crystals inside glass capillaries. The method is based upon the properties of gels, which are used to hold capillaries containing the protein solution, and also to act as the mass transport medium for the precipitating agent. Recipes for a set of selected experiments bringing a hands-on experience on thecrystallization of different soluble proteins are supplied. These experiments are inexpensive and straightforward enough for teaching at the undergraduate level that large biological macromolecules that are the gate our structural studies and drug design can be crystallized. Using simple equations accounting for the solubility of proteins and for the nucleation process, the experimental resultsare explained to provide a rational approach to the problem. In addition, because of the nature of the crystallization method which is based on diffusion-reactions systems, the student is introduced to the meaning of self-organization. (Am. Chem. Soc., 75: 442-446 Material Research Bulletin, 28: 541-546.)
En la estación espacial, en condiciones de microgravedad, se desarrollan los mejorescristales de proteínas
Cristales buenos sí, pero... ¿a qué precio y con cuantas dificultades?
Protein crystallization by the gel acupuncture method (Our method)
Solo los cristales obtenidos con nuestra técnica de crecimiento han sido capaces de competir en calidad y tamaño con los cristales del laboratorio espacial, pero a bajo precio y con la cómoda rutina...
La formación de proto-proteinas en los primeros estadios de la química prebiótica es un misterio sin resolver ¿Como pudo generarse el enlace peptídico entre los primeros aminoacidos en un ambiente geológico plausible?
Peptide bonds formation was attempted by thermal activation of dry amino acids from aqueous solution thatsimulated prebiotic evaporative environments. The evaporation trend of amino acids solutions shows a bifurcation and can lead to either a crystalline phase (near equilibrium) or a metastable-non crystalline phase (far from equilibrium). Only amino acids in this metastable phase are able to form peptide bonds by thermal activation at temperatures that are generated by solar radiation today. We suggestthat this metastable phase is the ideal initial material to trigger amino acid assemblage with protein-like structure because provide the driving force (supersaturation) for an intense interaction between monomers of different amino acids and allows activation of these monomers in plausible prebiotic conditions
Origins of Life and Evolution of the Biosphere, 30: 549-556. Viedma (2000)En este trabajo sintetizamos proto-proteinas en ambientes moderados y establecemos que la diferencia entre auto-organización cristalina y auto-organización orgánica (estado metastable) permite la formación del enlace peptídico.
Optical microphotographs (x12) of a set of drops from the same solution of aminoacids B after evaporation and ageing for a few days during the ageing process,we selected some crystallized drops, some metastable drops and another one partially metastable-crystalline drop. All them were heated at 85ºC for 80 days. Only aminoacids in metastable state formed peptide bonds.
CÓMO CRECER BUENOS CRISTALES DE PROTEÍNAS Y POR QUÉ
Para conocer la estructura tridimensional de una proteína y por tanto para conocer con exactitud sufunción biológica hay que obtener cristales para estudiarlos mediante rayos X. Obtener cristales de proteínas es muy difícil por la complejidad fisicoquímica de las interacciones a escala molecular. En este trabajo desarrollamos una técnica de cristalización de proteínas que soslaya todos los problemas planteados y ha generado los mejores cristales obtenidos hasta ahora en la TierraThis paper provides a detailed description of a simple method to obtain large protein single crystals inside glass capillaries. The method is based upon the properties of gels, which are used to hold capillaries containing the protein solution, and also to act as the mass transport medium for the precipitating agent. Recipes for a set of selected experiments bringing a hands-on experience on thecrystallization of different soluble proteins are supplied. These experiments are inexpensive and straightforward enough for teaching at the undergraduate level that large biological macromolecules that are the gate our structural studies and drug design can be crystallized. Using simple equations accounting for the solubility of proteins and for the nucleation process, the experimental resultsare explained to provide a rational approach to the problem. In addition, because of the nature of the crystallization method which is based on diffusion-reactions systems, the student is introduced to the meaning of self-organization. (Am. Chem. Soc., 75: 442-446 Material Research Bulletin, 28: 541-546.)
En la estación espacial, en condiciones de microgravedad, se desarrollan los mejorescristales de proteínas
Cristales buenos sí, pero... ¿a qué precio y con cuantas dificultades?
Protein crystallization by the gel acupuncture method (Our method)
Solo los cristales obtenidos con nuestra técnica de crecimiento han sido capaces de competir en calidad y tamaño con los cristales del laboratorio espacial, pero a bajo precio y con la cómoda rutina...
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