Páginas: 8 (1976 palabras) Publicado: 28 de septiembre de 2012
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Converting the Sample Manager to Install a Column Manager or Column Heater/Cooler
This procedure outlines the steps necessary to convert the ACQUITY UltraPerformanceLCTM System Sample Manager to install a column manager or column heater/cooler.1 The new sample manager configuration includes an autosampler with either a column manager or a column heater/cooler(Figure 1).

Figure 1 - ACQUITY UPLC System Stack with Column Manager or Column Heater/Cooler

NOTE: If you are installing or replacing the column manager or column heater/cooler, you must have ACQUITY 1.23 (or later) software installed on your computer.

1. To be certain you have the latest version of this procedure, please visit the Support Center at

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Parts Required
Tables 1 and 2 list the part numbers for each of the column compartments. Be sure to use the parts corresponding to the parts you want to install.
Table 1: Parts Required for Column Manager

Part Number 176001285

Description ACQUITY UPLC Column Manager Kit

Quantity 1

Table 2: Parts Required for Column Heater/Cooler

Part Number 176001287 205000291 205000365Description ACQUITY UPLC Column Heater/Cooler Kit Column Stabilizer Kit, 50/100 mm Column Stabilizer Kit, 150 mm

Quantity 1 1 1

Tools/Materials Required
• T20 Torx screwdriver • T10 Torx screwdriver • Procedure entitled “Installing the ACQUITY UPLC System in a Primary Stacked Configuration” (available in the Waters Support Center) For column manager installation only: • • • • • • New ACQUITYUPLCTM column Open-ended 5/16-inch wrench Waters collet separator, PN 400001829 Fitting wrench, PN 415001941 PEEK tubing cutter Optical magnifier (optional)

The conversion procedure consists of several steps: 1. Removing the column heater from the autosampler 2. Installing the new column compartment 3. Installing the column 4. Making the electrical connections 5. Installing thesoftware/firmware 6. Preparing the column for use Removing the Column Heater 1. 2. Remove power to the instrument. Disconnect the power cord and Ethernet cables from the instrument.


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3. 4.

Remove the detector from the stack. Disconnect the column heater module from the autosampler: a. Open the column heater door and remove thecolumn and column stabilizer. NOTE: The column stabilizer must be installed in the column heater/cooler only. Column stabilizers do not need to be installed into the column manager (they are already built into the instrument). b. With a T20 Torx screwdriver, remove the panel from the right side of the autosampler. c. Disconnect the column compartment connectors (J3 and J38) from the autosamplerpersonality PCB (Figure 2).

Figure 2 - Disconnecting the Column Heater Cables

d. Install the shorting connector (WAT072940) on pin 2 of the J8 connector (Figure 3).

Figure 3 - Installing the Shorting Connector on J8


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e. Cut the tie-wrap (or undo the P-clip) holding the cables to the autosampler (Figure 2). 5. Use a T20 Torxscrewdriver to loosen the captive screw on the back of the autosampler (Figure 4). 6. Remove the column heater (slide backwards and up). NOTE: If the foam in the top of the autosampler dislodges while removing the column heater, reposition it as shown in Figure 5.

Figure 4 - Loosening the Captive Screw

Figure 5 - Autosampler Foam (Correct Position)


Install the new flat cover onto theautosampler, sliding its tabs into the foam slots (Figure 5).


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8. Push the cover forward to lock it. 9. Retighten the captive screw on the back of the cover. 10. Reinstall the autosampler side panel, using the T20 Torx driver. Installing a Column Heater/Cooler NOTE: Follow the steps in this section if you are installing a column...
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