Geminivirus

Virology Journal
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Transcriptome analysis of symptomatic and recovered leaves of geminivirus-infected pepper (Capsicum annuum)
Virology Journal 2012, 9:295 doi:10.1186/1743-422X-9-295

Elsa Góngora-Castillo (egongora@ira.cinvestav.mx)Enrique Ibarra-Laclette (ilaclette@ira.cinvestav.mx) Diana Trejo-Saavedra (dtrejo@ira.cinvestav.mx) Rafael F Rivera-Bustamante (rrivera@ira.cinvestav.mx)

ISSN Article type Submission date Acceptance date Publication date Article URL

1743-422X Research 21 May 2012 21 November 2012 27 November 2012 http://www.virologyj.com/content/9/1/295

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Transcriptome analysis of symptomatic and recovered leaves of geminivirus-infected pepper(Capsicum annuum)
Elsa Góngora-Castillo1 Email: egongora@ira.cinvestav.mx Enrique Ibarra-Laclette2 Email: ilaclette@ira.cinvestav.mx Diana Trejo-Saavedra1 Email: dtrejo@ira.cinvestav.mx Rafael F Rivera-Bustamante1* * Corresponding author Email: rrivera@ira.cinvestav.mx
1

Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del I.P.N (Cinvestav)-Unidad Irapuato, Km9.6 Libramiento Norte, Carretera Irapuato-León, Irapuato, Gto. 36821, México
2

Laboratorio Nacional de Genómica para la Biodiversidad (Langebio), Cinvestav-Irapuato, Km 9.6 Libramiento Norte, Carretera Irapuato-León, Irapuato, Gto. 36821, México

Abstract
Background
Geminiviruses are a large and important family of plant viruses that infect a wide range of crops throughout the world. TheBegomovirus genus contains species that are transmitted by whiteflies and are distributed worldwide causing disease on an array of horticultural crops. Symptom remission, in which newly developed leaves of systemically infected plants exhibit a reduction in symptom severity (recovery), has been observed on pepper (Capsicum annuum) plants infected with Pepper golden mosaic virus (PepGMV). Previousstudies have shown that transcriptional and post-transcriptional gene silencing mechanisms are involved in the reduction of viral nucleic acid concentration in recovered tissue. In this study, we employed deep transcriptome sequencing methods to assess transcriptional variation in healthy (mock), symptomatic, and recovered pepper leaves following PepGMV infection.

Results
Differentialexpression analyses of the pepper leaf transcriptome from symptomatic and recovered stages revealed a total of 309 differentially expressed genes between healthy (mock) and symptomatic or recovered tissues. Computational prediction of differential expression was validated using quantitative reverse-transcription PCR confirming the robustness of our bioinformatic methods. Within the set of differentiallyexpressed genes

associated with the recovery process were genes involved in defense responses including pathogenesis-related proteins, reactive oxygen species, systemic acquired resistance, jasmonic acid biosynthesis, and ethylene signaling. No major differences were found when compared the differentially expressed genes in symptomatic and recovered tissues. On the other hand, a set of genes...